Elucidating cell surface glycan imbalance through SERS guided metabolic glycan labelling: An appraisal of metastatic potential in cancer cells

被引：5

作者：

Murali, Madhukrishnan ^{[1
,2
]}

Murali, Vishnu Priya ^{[1
]}

Joseph, Manu M. ^{[1
]}

Rajan, Soumya ^{[3
]}

Maiti, Kaustabh Kumar ^{[1
,2
]}

机构：

[1] CSIR NIIST, CSTD, Organ Chem Sect, Industrial Estate, Thiruvananthapuram 695019, Kerala, India

[2] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India

[3] Govt Coll, Kasargod 671123, Kerala, India

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2022年 / 234卷

关键词：

Metabolic labelling; SERS; Cell discrimination; Cancer; Metastatic potential; Glycan; REPORTER STRATEGY; GLYCOSYLATION; ALKYNE; QUANTIFICATION; IDENTIFICATION; VISUALIZATION; BIOMARKER; ANALOGS; PROBE;

D O I：

10.1016/j.jphotobiol.2022.112506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intrinsic complexities of cell-surface glycans impede tracking the metabolic changes in cells. By coupling metabolic glycan labelling (MGL) and surface-enhanced Raman scattering (SERS), we employed the MGL-SERS strategy to elucidate the differential glycosylation pattern in cancer cell lines. Herein, for the first time, we are reporting an N-alkyl derivative of glucosamine (GlcNPhAlk) as a glycan labelling precursor. The extent of labelling was assessed by utilizing Raman imaging and verified by complementary fluorescence and Western blot analysis. MGL-SERS technique was implemented for a comparative evaluation of cell surface glycan imbalance in different cancer cells wherein a linear relationship between glycan expression and metastatic potential was established. Further, the effect of sialyltransferase inhibitor, P-3Fax-Neu5Ac, on metabolic labelling of GlcNPhAlk proved the incorporation of GlcNPhAlk to the terminal glycans through the sialic acid biosynthetic pathway. Hence, this methodology unveils the phenomenon of metastatic progression in cancer cells with inherent glycosylation-related dysplasia.

引用

页数：10

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