Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae

被引：0
|
作者：
Krongdang, Sasiprapa [1 ,2 ]
Pettis, Jeffery S. [3 ]
Williams, Geoffrey R. [4 ]
Chantawannakul, Panuwan [1 ,5 ,6 ]
机构：
[1] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Lab, Chiang Mai 50200, Thailand
[2] Chiang Mai Univ, Grad Sch, Interdisciplinary Program Biotechnol, Chiang Mai 50200, Thailand
[3] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA
[4] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA
[5] Chiang Mai Univ, Ctr Excellence Bioresources Agr Ind & Med, Chiang Mai 50200, Thailand
[6] Chiang Mai Univ, Int Coll Digital Innovat, Chiang Mai 50200, Thailand
来源：
CHIANG MAI JOURNAL OF SCIENCE | 2018年 / 45卷 / 03期
关键词：
honey bee; 16S rRNA; microbial ecology; Paenibacillus larvae; antagonistic bacteria; GUT; INHIBITION; DIVERSITY;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P. larvae; Bacillus pocheonensis (VN101) showed the largest zone of inhibition (26 +/- 1 mm). In this study, the diversity and richness of antagonistic isolates indicated that Bacillus spp. are the most promising as inhibitors of P. larvae. These finding suggested that certain bacterial isolates can act as antagonist to control P. larvae and may have other biotechnological applications.
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页码：1202 / 1213
页数：12
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