Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering

被引：88

作者：

Li, Chunching ^{[1
,2
]}

Armstrong, James P. K. ^{[1
,2
]}

Pence, Isaac J. ^{[1
,2
]}

Kit-Anan, Worrapong ^{[1
,2
]}

Puetzer, Jennifer L. ^{[1
,2
]}

Carreira, Sara Correia ^{[3
]}

Moore, Axel C. ^{[1
,2
]}

Stevens, Molly M. ^{[1
,2
]}

机构：

[1] Imperial Coll London, Dept Mat, Dept Bioengn, Prince Consort Rd, London SW7 2AZ, England

[2] Imperial Coll London, Inst Biomed Engn, Prince Consort Rd, London SW7 2AZ, England

[3] Univ Bristol, HH Wills Phys Lab, Tyndall Ave, Bristol BS8 1TL, Avon, England

来源：

BIOMATERIALS | 2018年 / 176卷

基金：

英国工程与自然科学研究理事会; 英国惠康基金; 英国医学研究理事会; 欧洲研究理事会;

关键词：

Magnetic; Nanoparticles; Gradients; Osteochondral; Tissue engineering; MESENCHYMAL STEM-CELLS; BONE MORPHOGENETIC PROTEIN-2; IN-VITRO; DIFFERENTIATION; CARTILAGE; HYDROGELS; BETA; PROLIFERATION; INTERFACES; SCAFFOLDS;

D O I：

10.1016/j.biomaterials.2018.05.029

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function. Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs. In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems. Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage. Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes. This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges. (C) 2018 The Authors. Published by Elsevier Ltd.

引用

页码：24 / 33

页数：10