Heparan sulfates and the decrease of N-glycans promote early adipogenic differentiation rather than myogenesis of murine myogenic progenitor cells

被引：2

作者：

Grassot, Vincent ^{[1
,2
]}

Bouchatal, Amel ^{[1
,2
]}

Da Silva, Anne ^{[1
,2
]}

Chantepie, Sandrine ^{[3
,4
]}

Papy-Garcia, Duke ^{[3
,4
]}

Maftah, Abderrahman ^{[1
,2
]}

Gallet, Paul-Francois ^{[1
,2
]}

Petit, Jean-Michel ^{[1
,2
]}

机构：

[1] INRA, UMR 1061, F-87060 Limoges, France

[2] Univ Limoges, Fac Sci & Tech, UGMA, F-87060 Limoges, France

[3] CNRS, EAC 7149, F-94000 Creteil, France

[4] Univ Paris Est Creteil, Lab Croissance Regenerat Reparat & Regenerat Tiss, CRRET, F-94000 Creteil, France

来源：

DIFFERENTIATION | 2017年 / 93卷

关键词：

Glycosylation related genes; Murine myogenic progenitor cells (MPC); Early adipogenesis; Myogenesis Heparan sulfates; N-glycan sialylation; MUSCLE SATELLITE CELLS; FIBROBLAST-GROWTH-FACTOR; SKELETAL-MUSCLE; STEM-CELLS; MYOBLAST DIFFERENTIATION; EXTRACELLULAR-MATRIX; TRANSFORMING GROWTH-FACTOR-BETA-1; ADIPOCYTE DIFFERENTIATION; SIGNALING PATHWAY; EXPRESSION;

D O I：

10.1016/j.diff.2016.08.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In vitro, extracted muscle satellite cells, called myogenic progenitor cells, can differentiate either in myotubes or preadipocytes, depending on environmental factors and the medium. Transcriptomic analyses on glycosylation genes during satellite cells differentiation into myotubes showed that 31 genes present a significant variation of expression at the early stages of murine myogenic progenitor cells (MPC) differentiation. In the present study, we analyzed the expression of 383 glycosylation related genes during murine MPC differentiation into preadipocytes and compared the data to those previously obtained during their differentiation into myotubes. Fifty-six glycosylation related genes are specifically modified in their expression during early adipogenesis. The variations correspond mainly to: a decrease of N-glycans, and of alpha (2,3) and (2,6) linked sialic acids, and to a high level of heparan sulfates. A high amount of TGF-beta 1 in extracellular media during early adipogenesis was also observed. It seems that the increases of heparan sulfates and TGF-beta 1 favor pre-adipogenic differentition of MPC and possibly prevent their myogenic differentiation.

引用

页码：15 / 26

页数：12