Calcium induced growth, physio-biochemical, antioxidants, osmolytes adjustments and phytoconstituents status in spinach under heat stress

被引:12
作者
Zaman, Qamar Uz [1 ]
Abbasi, Asim [2 ]
Tabassum, Sobia [3 ]
Ashraf, Kamran [4 ]
Ahmad, Zahoor [5 ]
Siddiqui, Manzer H. [6 ]
Alamri, Saud
Maqsood, Sumaira [2 ]
Sultan, Khawar [1 ]
机构
[1] Univ Lahore, Dept Environm Sci, Lahore 54590, Pakistan
[2] Kohsar Univ, Dept Environm Sci, Murree 47150, Pakistan
[3] Islamia Univ Bahawalpur, Fac Pharm, Dept Pharmaceut Chem, Bahawalpur 63100, Pakistan
[4] Govt Coll Univ Faisalabad, Dept Food Sci & Nutr, Sahiwal Campus, Sahiwal 57000, Pakistan
[5] Univ Campus, Punjab Grp Coll, Dept Bot, Bahawalpur 63100, Pakistan
[6] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
关键词
Antioxidant activities; Growth; Physio-biochemical; Osmolytes status; Thermo-protection; Spinach; Phytoconstituents; HIGH-TEMPERATURE STRESS; OXIDATIVE STRESS; WATER RELATIONS; SALICYLIC-ACID; TOLERANCE; MECHANISMS; METABOLISM; SALINITY; PLANTS; MAIZE;
D O I
10.1016/j.sajb.2022.06.065
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
High temperatures profoundly limit the growth and productivity of leafy vegetables. Investigating heatresponsive morpho-physiological and biochemical mechanisms is an important tool for the thermo-tolerance of crops. This research work is about the response of varying levels of exogenous Ca2+ supplementation (0, 2, 4, and 6 mmol L-1) on growth & development, gaseous exchange, antioxidant enzyme activities, and osmolytes adjustments in spinach plants grown under heat related stress. A rise in temperatures from 25 to 35 degrees C decreased leaf area, number of leaves, chlorophyll contents, and leaf fresh biomass of spinach by 34.20%, 26.88%, 21.73%, and 24.68%, respectively. Meanwhile, Ca2+ treated plants showed higher antioxidant enzyme activities, soluble sugar levels, and lower membrane leakage induced by heat stress. Leaf osmotic potential values of seedlings treated with the Ca2+ solutions were measured to be lower than the control. Under heat stress, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) significantly increased in spinach leaves. Exogenous Ca2+ supplementation enhanced heat tolerance in spinach by the maintenance of physio-biochemical, and a decrease in membrane lipid peroxidation and presence of phytoconstituents. Thus, an appreciable level of thermo-protection was induced by 4 mmol L-1 Ca2+ under heat stress conditions, indicating that the Ca2+ level required for growth under heat stress exceeds that required level for normal growth, regulation of enzymatic antioxidants, and osmolytes adjustments under normal conditions.
引用
收藏
页码:701 / 711
页数:11
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