Revisiting Optical Clearing With Dimethyl Sulfoxide (DMSO)

被引：57

作者：

Bui, Albert K. ^{[2
]}

McClure, R. Anthony ^{[2
,3
]}

Chang, Jennell ^{[2
]}

Stoianovici, Charles ^{[4
]}

Hirshburg, Jason ^{[6
]}

Yeh, Alvin T. ^{[6
]}

Choi, Bernard ^{[1
,5
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Sch Biol Sci, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Dept Neurobiol, Irvine, CA 92697 USA

[4] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[5] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

[6] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 USA

来源：

LASERS IN SURGERY AND MEDICINE | 2009年 / 41卷 / 02期

基金：

美国国家科学基金会;

关键词：

glycerol; hyperosmotic agents; collagen dissociation; collagen solubility; integrating spheres; HUMAN SKIN; PENETRATION ENHANCERS; IN-VITRO; TISSUE; COLLAGEN; AGENTS; TOMOGRAPHY; MICROSCOPY; CONTRAST; DELIVERY;

D O I：

10.1002/lsm.20742

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Functional optical characterization of disease progression and response to therapy suffers from loss of spatial resolution and imaging depth due to scattering. Here we report on the ability of dimethyl sulfoxide (DMSO) alone to reduce the optical scattering of skin. We observed a threefold reduction in the scattering of skin with topical DMSO application. With an in vivo window chamber model, we observed a threefold increase in light transmittance through the preparation and enhanced visualization of subsurface microvasculature. Collectively, our data demonstrate the potential of DMSO alone to mitigate effects of scattering, which we expect will improve molecular imaging studies. Lasers Surg. Med. 41:142-148, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：142 / 148

页数：7

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