CryIIIA toxin gene expression in transgenic rice confers resistance to rice water weevil

被引:3
作者
Lee, Jin-Hyoung [1 ]
Shin, Kong-Sik [1 ]
Suh, Seok-Cheol [1 ]
Rhim, Seong-Lyul [2 ]
Lee, Yeon-Hee [1 ]
Lim, Myung-Ho [1 ]
Woo, Hee-Jong [1 ]
Qin, Yang [1 ]
Cho, Hyun-Suk [1 ]
机构
[1] Natl Acad Agr Sci, Rural Dev Adm, Suwon 441707, Gyeonggi Do, South Korea
[2] Inwoo Cooperat Ltd, Seoul 130050, South Korea
关键词
cryIIIA; Insect bioassay; Rice water weevil; Transgenic rice; LISSORHOPTRUS-ORYZOPHILUS KUSCHEL; POPULATION-DYNAMICS; TRYPSIN-INHIBITOR; SYNTHETIC CRY3A; PORE FORMATION; COLEOPTERA; CURCULIONIDAE; MANAGEMENT; TRANSFORMATION; AGROBACTERIUM;
D O I
10.1007/s11240-013-0356-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel, is the most widely distributed and destructive early season insect pest of rice, Oryza sativa L. worldwide. The rice plants were transformed with cryIIIA insecticidal gene as well as with the bar gene coding phosphinothricin acetyltransferase. CryIIIA gene under the control of a modified RCg2 promoter drives the insect-toxic gene expression in roots and/or leaves. The cryIIIA gene was transferred into O. sativa L. cv. Nakdong by Agrobacterium-mediated transformation. Stable integration of the transgene was confirmed in putative transformed rice by Southern blot analysis. The expression of the cryIIIA toxin gene in the roots of transgenic rice plants was verified by RT-PCR and immunoblot analysis. Transgenic rice plants were also evaluated for resistance to natural infestations of the RWW under field conditions between 2007 and 2011. The transgenic Btt8R and Btt12R lines reduced the growth rate of RWW larvae and pupae populations compared with non-transgenic control plants by approximately 52 and 58 %, respectively. To further examine the efficacy of the RWW bioassay, we used pots and performed experiments in trays and under field conditions in 2012. The Btt12R line reduced the total populations of RWW larvae and pupae in trays and under field conditions by 56 and 45 %, respectively. The bioassay experiments conducted over 6 years, showed a significant reduction rate of RWW larvae and pupae populations demonstrating that the cryIIIA gene in transgenic rice confers resistance to RWW.
引用
收藏
页码:243 / 252
页数:10
相关论文
共 45 条
[41]   CHARACTERIZATION OF A RICE GENE FAMILY ENCODING ROOT-SPECIFIC PROTEINS [J].
XU, Y ;
BUCHHOLZ, WG ;
DEROSE, RT ;
HALL, TC .
PLANT MOLECULAR BIOLOGY, 1995, 27 (02) :237-248
[42]   Laboratory and field evaluation of the transgenic Populus alba x Populus glandulosa expressing double coleopteran-resistance genes [J].
Zhang, Bingyu ;
Chen, Min ;
Zhang, Xiaofen ;
Luan, Hehui ;
Diao, Shu ;
Tian, Yingchuan ;
Su, Xiaohua .
TREE PHYSIOLOGY, 2011, 31 (05) :567-573
[43]   Evaluation of the resistance of transgenic potato plants expressing various levels of Cry3A against the Colorado potato beetle (Leptinotarsa decemlineata Say) in the laboratory and field [J].
Zhou, Zhaoxu ;
Pang, Jinhuan ;
Guo, Wenchao ;
Zhong, Naiqin ;
Tian, Yingchuan ;
Xia, Guixian ;
Wu, Jiahe .
PEST MANAGEMENT SCIENCE, 2012, 68 (12) :1595-1604
[44]   The effects of feeding by the rice water weevil, Lissorhoptrus oryzophilus Kuschel, on the growth and yield components of rice, Oryza sativa [J].
Zou, L ;
Stout, MJ ;
Dunand, RT .
AGRICULTURAL AND FOREST ENTOMOLOGY, 2004, 6 (01) :47-53
[45]   Density-yield relationships for rice water weevil on rice for different varieties and under different water management regimes [J].
Zou, L ;
Stout, MJ ;
Ring, DR .
CROP PROTECTION, 2004, 23 (06) :543-550