First Gene Cassettes of Integrons as Targets in Finding Adaptive Genes in Metagenomes

被引:12
|
作者
Huang, Lionel [1 ]
Cagnon, Christine [1 ]
Caumette, Pierre [1 ]
Duran, Robert [1 ]
机构
[1] Univ Pau & Pays Adour, CNRS, UMR 5254, Equipe Environm & Microbiol,IPREM,IBEAS, F-64013 Pau, France
关键词
ANTIBIOTIC-RESISTANCE GENES; ENVIRONMENTAL DNA; CLASS-1; INTEGRONS; VIBRIO-CHOLERAE; RECOMBINATION; IDENTIFICATION; DIVERSITY; EVOLUTION; RECOVERY; BACTERIA;
D O I
10.1128/AEM.02394-08
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The first gene cassettes of integrons are involved in the last adaptation response to changing conditions and are also the most expressed. We propose a rapid method for the selection of clones carrying an integron first gene cassette that is useful for finding adaptive genes in environmental metagenomic libraries.
引用
收藏
页码:3823 / 3825
页数:3
相关论文
共 50 条
  • [1] Integrons and gene cassettes: hotspots of diversity in bacterial genomes
    Hall, Ruth M.
    EFFECTS OF GENOME STRUCTURE AND SEQUENCE ON VARIATION AND EVOLUTION, 2012, 1267 : 71 - 78
  • [2] Impact of Wastewater Treatment on the Prevalence of Integrons and the Genetic Diversity of Integron Gene Cassettes
    An, Xin-Li
    Chen, Qing-Lin
    Zhu, Dong
    Zhu, Yong-Guan
    Gillings, Michael R.
    Su, Jian-Qiang
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2018, 84 (09)
  • [3] Methods for the targeted sequencing and analysis of integrons and their gene cassettes from complex microbial communities
    Ghaly, Timothy M.
    Penesyan, Anahit
    Pritchard, Alexander
    Qi, Qin
    Rajabal, Vaheesan
    Tetu, Sasha G.
    Gillings, Michael R.
    MICROBIAL GENOMICS, 2022, 8 (03):
  • [4] Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
    Waldron, Liette S.
    Gillings, Michael R.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (100):
  • [5] Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays
    Elsaied, Hosam
    Stokes, Hatch W.
    Kitamura, Keiko
    Kurusu, Yasurou
    Kamagata, Yoichi
    Maruyama, Akihiko
    ISME JOURNAL, 2011, 5 (07): : 1162 - 1177
  • [6] Diverse aadA gene cassettes on class 1 integrons introduced into soil via spread manure
    Binh, Chu T. T.
    Heuer, Holger
    Kaupenjohann, Martin
    Smalla, Kornelia
    RESEARCH IN MICROBIOLOGY, 2009, 160 (06) : 427 - 433
  • [7] Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core
    Elsaied, Hosam
    Stokes, Hatch W.
    Yoshioka, Hideyoshi
    Mitani, Yasuo
    Maruyama, Akihiko
    FEMS MICROBIOLOGY ECOLOGY, 2014, 87 (02) : 343 - 356
  • [8] Mobile gene cassettes and integrons: Moving antibiotic resistance genes in Gram-negative bacteria
    Hall, RM
    ANTIBIOTIC RESISTANCE: ORIGINS, EVOLUTION, SELECTION AND SPREAD, 1997, 207 : 192 - 202
  • [9] Evidence for dynamic exchange of qac gene cassettes between class 1 integrons and other integrons in freshwater biofilms
    Gillings, Michael R.
    Holley, Marita P.
    Stokes, H. W.
    FEMS MICROBIOLOGY LETTERS, 2009, 296 (02) : 282 - 288
  • [10] INTEGRALL: a database and search engine for integrons, integrases and gene cassettes
    Moura, Alexandra
    Soares, Mario
    Pereira, Carolina
    Leitao, Nuno
    Henriques, Isabel
    Correia, Antonio
    BIOINFORMATICS, 2009, 25 (08) : 1096 - 1098