A correlation between capsid protein VP2 and the plaque morphology of African horse sickness virus in cell culture

被引:4
作者
Schade-Weskott, Mathilde L. [1 ,2 ]
van Schalkwyk, Antoinette [1 ]
Koekemoer, J. J. O. [1 ]
机构
[1] Agr Res Council, Onderstepoort Vet Inst, 100 Old Soutpan Rd, Pretoria, South Africa
[2] Univ Pretoria, Dept Vet Trop Dis, Fac Vet Sci, Pretoria, South Africa
关键词
African horse sickness virus; Attenuation; Plaque phenotype; VP2-gene; BLUETONGUE VIRUS; HORSESICKNESS-VIRUS; NONSTRUCTURAL PROTEIN; RECOMBINANT BACULOVIRUS; SEQUENCE-ANALYSIS; POLYMERASE GENE; INSECT CELLS; RNA; IDENTIFICATION; LOCALIZATION;
D O I
10.1007/s11262-018-1567-y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The attenuated live virus vaccine that is used in South Africa to protect against African horse sickness infection was developed more than 50 years ago. With the selection of the vaccine strains by cell culture passage, a correlation between the size of plaques formed in monolayer Vero cultures and attenuation of virus virulence in horses was found. The large plaque phenotype was used as an indication of cell culture adaptation and strongly correlated with attenuation of virulence in horses. There was never any investigation into the genetic causes of either the variation in plaque size, or the loss of virulence. An understanding of the underlying mechanisms of attenuation would benefit the production of a safer AHSV vaccine. To this end, the genomes of different strains of two African horse sickness isolates, producing varying plaque sizes, were compared and the differences between them identified. This comparison suggested that proteins VP2, VP3, VP5 and NS3 were most likely involved in the determination of the plaque phenotype. Comparison between genome sequences (obtained from GenBank) of low and high passage strains from two additional serotypes indicated that VP2 was the only protein with amino acid substitutions in all four serotypes. The amino acid substitutions all occurred within the same hydrophilic area, resulting in increased hydrophilicity of VP2 in the large plaque strains.
引用
收藏
页码:527 / 535
页数:9
相关论文
共 61 条
  • [1] Abramoff M.D., 2004, Biophotonics Int., V11, P36
  • [2] ExPASy: SIB bioinformatics resource portal
    Artimo, Panu
    Jonnalagedda, Manohar
    Arnold, Konstantin
    Baratin, Delphine
    Csardi, Gabor
    de Castro, Edouard
    Duvaud, Severine
    Flegel, Volker
    Fortier, Arnaud
    Gasteiger, Elisabeth
    Grosdidier, Aurelien
    Hernandez, Celine
    Ioannidis, Vassilios
    Kuznetsov, Dmitry
    Liechti, Robin
    Moretti, Sebastien
    Mostaguir, Khaled
    Redaschi, Nicole
    Rossier, Gregoire
    Xenarios, Ioannis
    Stockinger, Heinz
    [J]. NUCLEIC ACIDS RESEARCH, 2012, 40 (W1) : W597 - W603
  • [3] Detection of a Fourth Orbivirus Non-Structural Protein
    Belhouchet, Mourad
    Jaafar, Fauziah Mohd
    Firth, Andrew E.
    Grimes, Jonathan M.
    Mertens, Peter P. C.
    Attoui, Houssam
    [J]. PLOS ONE, 2011, 6 (10):
  • [4] AN ATTEMPT TO DEFINE THE HOST RANGE FOR AFRICAN HORSE SICKNESS VIRUS (ORBIVIRUS, REOVIRIDAE) IN EAST-AFRICA, BY A SEROLOGICAL SURVEY IN SOME EQUIDAE, CAMELIDAE, LOXODONTIDAE AND CARNIVORE
    BINEPAL, VS
    WARIRU, BN
    DAVIES, FG
    SOI, R
    OLUBAYO, R
    [J]. VETERINARY MICROBIOLOGY, 1992, 31 (01) : 19 - 23
  • [5] BLACKBURN NK, 1985, J ENTOMOL SOC S AFR, V48, P331
  • [6] Purified recombinant bluetongue virus VP1 exhibits RNA replicase activity
    Boyce, M
    Wehrfritz, J
    Noad, R
    Roy, P
    [J]. JOURNAL OF VIROLOGY, 2004, 78 (08) : 3994 - 4002
  • [7] Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis
    Boyce, Mark
    Celma, Cristina C. P.
    Roy, Polly
    [J]. VIROLOGY JOURNAL, 2012, 9
  • [8] BREMER C W, 1976, Onderstepoort Journal of Veterinary Research, V43, P193
  • [9] CHARACTERIZATION AND CLONING OF THE AFRICAN HORSESICKNESS VIRUS GENOME
    BREMER, CW
    HUISMANS, H
    VANDIJK, AA
    [J]. JOURNAL OF GENERAL VIROLOGY, 1990, 71 : 793 - 799
  • [10] Calisher CH, 1998, ARCH VIROL, P3