Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

被引：6

作者：

Browning, Clare F. J. ^{[1
]}

Di Nardo, Antonello ^{[1
]}

Henry, Lissie ^{[1
]}

Pollard, Tim ^{[2
]}

Hendry, Lynne ^{[2
]}

Romey, Aurore ^{[3
]}

Relmy, Anthony ^{[3
]}

Eble, Phaedra ^{[5
]}

Brocchi, Emiliana ^{[4
]}

Grazioli, Santina ^{[4
]}

King, Donald P. ^{[1
]}

Ludi, Anna B. ^{[1
]}

机构：

[1] Pirbright Inst, Ash Rd, Surrey GU24 0NF, England

[2] Anim & Plant Hlth Agcy, New Haw, England

[3] Univ Paris Est, Ecole Natl Vet Alfort, ANSES, Lab Sante Anim Maisons Alfort,INRA, Maisons Alfort, France

[4] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Brescia, Italy

[5] Wageningen Biovet Res WBVR, Lelystad, Netherlands

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2020年 / 32卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

ELISA; foot-and-mouth disease virus; inter-laboratory study; serology; SPECIFICITY; SENSITIVITY;

D O I：

10.1177/1040638720962070

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0-95.2%) and 94.8% (95% CI: 93.1-96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

引用

页码：933 / 937

页数：5

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