Tannic Acid Induction of a Glutathione S-transferase in Micromelalopha troglodyta (Lepidoptera: Notodontidae) Larvae

被引:0
作者
Cheng, Huiping [1 ]
Tang, Fang [1 ]
Li, Wei [1 ]
Xu, Meng [1 ]
机构
[1] Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Jiangsu, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Micromelalopha troglodyta; tannic acid; glutathione S-transferases; MtGSTd1; mRNA expression; ANOPHELES-GAMBIAE; DROSOPHILA-MELANOGASTER; SPRUCE BUDWORM; FALL ARMYWORM; BOMBYX-MORI; EXPRESSION; CLONING; IDENTIFICATION; GENE; LOCALIZATION;
D O I
暂无
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Micromelalopha troglodyte (Graeser) (Lepidoptera: Notodontidae) is an important pest of poplar trees, Populus spp. (family Salicaceae), in China. Herein, we report the first cloning and sequencing of a full-length cDNA of a delta class (Class I) glutathione S-transferase (GST), MtGSTd1, from the larval midgut of M. troglodyte. The open reading frame of the MtGSTd1 cDNA was 657 bp and encoded 219 amino acid residues in M. troglodyte. Furthermore, specific activities of GSTs were induced in fat bodies and midguts of M. troglodyte by tannic acid. MtGSTd1 nnRNA also was induced by tannic acid in the fat bodies and midguts. GST activities increased following the elevated expression of GST nnRNA in M. troglodyte. These findings indicate that the GSTs may have an antioxidant role in the metabolism of plant secondary substances in M. troglodyte larvae.
引用
收藏
页码:350 / 362
页数:13
相关论文
共 34 条
[1]   Structure of a Drosophila sigma class glutathione S-transferase reveals a novel active site topography suited for lipid peroxidation products [J].
Agianian, B ;
Tucker, PA ;
Schouten, A ;
Leonard, K ;
Bullard, B ;
Gros, P .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 326 (01) :151-165
[2]  
Board PG, 1997, BIOCHEM J, V328, P929
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
Chelvanayagam G, 2001, CHEM-BIOL INTERACT, V133, P256
[5]  
Chen F., 2003, Ph.D. Thesis
[6]  
Chen Feng-Ju, 2003, Acta Entomologica Sinica, V46, P684
[7]   THE COMPARATIVE ENZYMOLOGY OF THE GLUTATHIONE S-TRANSFERASES FROM NON-VERTEBRATE ORGANISMS [J].
CLARK, AG .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1989, 92 (03) :419-446
[8]   EVIDENCE THAT DDT-DEHYDROCHLORINASE FROM THE HOUSEFLY IS A GLUTATHIONE S-TRANSFERASE [J].
CLARK, AG ;
SHAMAAN, NA .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 1984, 22 (03) :249-261
[9]   The Anopheles gambiae glutathione transferase supergene family:: annotation, phylogeny and expression profiles -: art. no. 35 [J].
Ding, YC ;
Ortelli, F ;
Rossiter, LC ;
Hemingway, J ;
Ranson, H .
BMC GENOMICS, 2003, 4 (1)
[10]   Glutathione S-transferase from the spruce budworm, Choristoneura fumiferana:: identification, characterization, localization, cDNA cloning, and expression [J].
Feng, QL ;
Davey, KG ;
Pang, ASD ;
Primavera, M ;
Ladd, TR ;
Zheng, SC ;
Sohi, SS ;
Retnakaran, A ;
Palli, SR .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 29 (09) :779-793