Determination of the deoxycytidine with a non-radiochemical B kinase activity in cell homogenates assay using reversed-phase high performance liquid chromatography - Identification of a novel metabolite of 2-chlorodeoxyadenosine

A non-radioactive procedure to measure the deoxycytidine kinase (dCK) activity in crude cell free homogenates was developed. 2-Chlorodeoxyadenosine (CdA) was used as the substrate for dCK and was separated from its product 2-chlorodeoxyadenosine-5'-monophosphate (CdAMP) by reversed-phase HPLC. A complete separation of CdA and its metabolites was achieved in 30 min. The minimum amount of CdAMP that could be detected was 1 pmol. The assay was linear with reaction times up to at least 3 h. With respect to the protein concentration, the reaction was linear with protein concentrations up to 760 mug/ml in the assay. An amount of 8 x 103 cells was already sufficient to determine the specific dCK activity in SK-N-BE(2)c cells. CdA was not only converted to CdAMP but also to 2-chloroadenine and, surprisingly, also to 2-chlorodeoxyinosine, in MOLT-3 cells. The deamination of CdA was completely inhibited by deoxycoformycin, which clearly demonstrates that CdA is a substrate for adenosine deaminase. (C) 2004 Elsevier B.V. All rights reserved.