Optimal lens design and use in laser-scanning microscopy

被引：50

作者：

Negrean, Adrian ^{[1
,2
]}

Mansvelder, Huibert D. ^{[1
,2
]}

机构：

[1] Vrije Univ Amsterdam, Ctr Neurogen & Cognit Res, NL-1081 HV Amsterdam, Netherlands

[2] Neurosci Campus Amsterdam, NL-1081 HV Amsterdam, Netherlands

来源：

BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 05期

关键词：

2-PHOTON MICROSCOPY; TISSUE;

D O I：

10.1364/BOE.5.001588

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In laser-scanning microscopy often an off-the-shelf achromatic doublet is used as a scan lens which can reduce the available diffraction-limited field-of-view (FOV) by a factor of 3 and introduce chromatic aberrations that are scan angle dependent. Here we present several simple lens designs of superior quality that fully make use of high-NA low-magnification objectives, offering diffraction-limited imaging over a large FOV and wavelength range. We constructed a two-photon laser-scanning microscope with optimized custom lenses which had a near diffraction limit point-spread-function (PSF) with less than 3.6% variation over a 400 mu m FOV and less than 0.5 mu m lateral color between 750 and 1050 nm. (C) 2014 Optical Society of America

引用

页码：1588 / 1609

页数：22

共 20 条

[1] Practical limits of resolution in confocal and non-linear microscopy [J].