DNMT3B modulates the expression of cancer-related genes and downregulates the expression of the gene VAV3 via methylation

被引：0
作者：
Peralta-Arrieta, Irlanda [1 ]
Hernandez-Sotelo, Daniel [2 ]
Castro-Coronel, Yaneth [3 ]
Antonio Leyva-Vazquez, Marco [1 ]
Illades-Aguiar, Berenice [1 ]
机构：
[1] Univ Autonoma Guerrero, Fac Ciencias Quim Biol, Lab Biomed Mol, Ave Lazaro Cardenas,Ciudad Univ, Chilpancingo 39090, Guerrero, Mexico
[2] Univ Autonoma Guerrero, Fac Ciencias Quim Biol, Lab Epigenet Canc, Chilpancingo, Guerrero, Mexico
[3] Univ Autonoma Guerrero, Fac Ciencias Quim Biol, Lab Invest Citopatol & Histoquim, Chilpancingo, Guerrero, Mexico
来源：
AMERICAN JOURNAL OF CANCER RESEARCH | 2017年 / 7卷 / 01期
关键词：
Methylation; de novo methyltransferase; overexpression of DNMT3B; cancer; cancer-related genes; VAV3; CpG island; COUPLED RECEPTOR 137; PERICENTROMERIC SATELLITE REGIONS; PUTATIVE TUMOR-SUPPRESSOR; DNA METHYLTRANSFERASE 3B; FIBROBLAST-GROWTH-FACTOR; BREAST EPITHELIAL-CELLS; TOPOISOMERASE-I-BINDING; DE-NOVO METHYLATION; OVARIAN-CANCER; CERVICAL-CANCER;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Altered promoter DNA methylation is one of the most important epigenetic abnormalities in human cancer. DNMT3B, de novo methyltransferase, is clearly related to abnormal methylation of tumour suppressor genes, DNA repair genes and its overexpression contributes to oncogenic processes and tumorigenesis in vivo. The purpose of this study was to assess the effect of the overexpression of DNMT3B in HaCaT cells on global gene expression and on the methylation of selected genes to the identification of genes that can be target of DNMT3B. We found that the overexpression of DNMT3B in HaCaT cells, modulate the expression of genes related to cancer, downregulated the expression of 151 genes with CpG islands and downregulated the expression of the VAV3 gene via methylation of its promoter. These results highlight the importance of DNMT3B in gene expression and human cancer.
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页码：77 / +
页数：28
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