Effects of various photoperiods on Kisspeptin and reproductive hormones in the goldfish, Carassius auratus

被引：9

作者：

Shin, Hyun Suk ^{[1
]}

Song, Jin Ah ^{[1
]}

Choi, Ji Yong ^{[1
]}

Kim, Na Na ^{[1
]}

Choi, Young Jae ^{[1
]}

Sung, Si Nae ^{[1
]}

Park, Mi Seon ^{[2
]}

Min, Byung Hwa ^{[2
]}

Choi, Cheol Young ^{[1
]}

机构：

[1] Korea Maritime & Ocean Univ, Div Marine Environm & BioSci, Pusan 606791, South Korea

[2] Natl Fisheries Res & Dev Inst, Aquaculture Management Div, Pusan 619705, South Korea

来源：

ANIMAL CELLS AND SYSTEMS | 2014年 / 18卷 / 02期

关键词：

photoperiod; gonadotropin-releasing hormone; kisspeptin; sex maturation; sex steroid hormone; GONADOTROPIN-RELEASING-HORMONE; EUROPEAN SEA BASS; DICENTRARCHUS-LABRAX; LUTEINIZING-HORMONE; SEASONAL-VARIATIONS; ATLANTIC SALMON; OOCYTE MEIOSIS; MESSENGER-RNA; GROWTH; KISS-1;

D O I：

10.1080/19768354.2014.902863

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The study aimed to test differences in the hormones of the hypothalamus-pituitary-gonad axis, Kisspeptin (Kiss) and sex steroids, and the sexual maturation of goldfish (Carassius auratus) according to various photoperiods: 10 h light (L):14 h dark (D) (short day), 12L:12D (control), and 14L:10D (long day). To investigate the sexual maturation under various photoperiods, quantitative real-time PCR (QPCR) assays for salmon gonadotropin-releasing hormone, chicken gonadotropin-releasing hormone, gonadotropin hormones (GTHs), Kisspeptin 1 (Kiss1), and its receptor, G protein-coupled receptor 54 (GPR54), were developed. mRNA expression was monitored in cultured pituitary cells (in vitro) of various photoperiod groups. Furthermore, we injected or treated the animals with Kiss and found that Kiss treatment increases the mRNA expression levels of GTHs. We measured the plasma follicle-stimulating hormone (FSH), luteinizing hormone (LH), and 17 alpha-hydroxypregnenolone levels. The gonadotropin-releasing hormones (GnRHs), GTHs, Kiss1/GPR54 mRNA expression, plasma FSH, LH, and 17 alpha-hydroxypregnenolone levels in the 14L:10D group were significantly higher than in the other groups. These results suggest that light length regulates sex maturation by GnRH and Kiss1 in the brain of the goldfish.

引用

页码：109 / 118

页数：10

共 45 条

[1] Three forms of gonadotropin-releasing hormone, including a novel form, in a basal salmonid, Coregonus clupeaformis [J].