Synthesis of pH-Responsive Chitosan Nanocapsules for the Controlled Delivery of Doxorubicin

被引：51

作者：

Chen, Chih-Kuang ^{[1
,3
]}

Wang, Qing ^{[1
]}

Jones, Charles H. ^{[1
]}

Yu, Yun ^{[1
]}

Zhang, Hanguang ^{[1
]}

Law, Wing-Cheung ^{[2
]}

Lai, Cheng Kee ^{[1
]}

Zeng, Qinghang ^{[1
]}

Prasad, Paras N. ^{[2
]}

Pfeifer, Blaine A. ^{[1
]}

Cheng, Chong ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Chem & Biol Engn, Buffalo, NY 14260 USA

[2] SUNY Buffalo, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

[3] Feng Chia Univ, Dept Fiber & Composite Mat, Taichung 40724, Taiwan

来源：

LANGMUIR | 2014年 / 30卷 / 14期

基金：

美国国家科学基金会;

关键词：

DRUG-DELIVERY; BIODEGRADABLE NANOPARTICLES; GENE DELIVERY; CANCER-CELLS; POLYMERIC NANOPARTICLES; MULTIDRUG-RESISTANCE; CROSS-LINKING; CO-DELIVERY; CARRIERS; TUMOR;

D O I：

10.1021/la4040485

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Well-defined chitosan nanocapsules (CSNCs) with tunable sizes were synthesized through the interfacial cross-linking of N-maleoyl-functionalized chitosan (MCS) in miniemulsions, and their application in the delivery of doxorubicin (Dox) was investigated. MCS was prepared by the amidation reaction of CS with maleic anhydride in water/DMSO at 65 degrees C for 20 h. Subsequently, thiol-ene cross-linking was conducted in oil-in-water miniemulsions at room temperature under UV irradiation for 1 h, using MCS as both a surfactant and precursor polymer, 1,4-butanediol bis(3-mercapto-propionate) as a cross-linker, and D-alpha-tocopheryl poly(ethylene glycol) 1000 succinate as a cosurfactant. With the increase in cosurfactant concentration in the reaction systems, the sizes of the resulting CSNCs decreased steadily. Dox-loaded CSNCs were readily prepared by in situ encapsulation of Dox during miniemulsion cross-linking. With acid-labile beta-thiopropionate cross-linkages, the Dox-loaded CSNCs demonstrated a faster release rate under acidic conditions. Relative to free Dox, Dox-loaded CSNCs exhibited enhanced cytotoxicity toward MCF-7 breast cancer cells without any noticeable cytotoxicity from empty CSNCs. The effective delivery of Dox to MCF-7 breast cancer cells via Dox-loaded CSNCs was also observed.

引用

页码：4111 / 4119

页数：9

共 59 条

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