Prediction of anticancer peptides against MCF-7 breast cancer cells from the peptidomes of Achatina fulica mucus fractions

被引：69

作者：

E-kobon, Teerasak ^{[1
]}

Thongararm, Pennapa ^{[2
]}

Roytrakul, Sittiruk ^{[3
]}

Meesuk, Ladda ^{[4
]}

Chumnanpuen, Pramote ^{[2
]}

机构：

[1] Kasetsart Univ, Dept Genet, Fac Sci, Bangkok 10900, Thailand

[2] Kasetsart Univ, Dept Zool, Fac Sci, 50 Ngam Wong Wan Rd, Bangkok 10900, Thailand

[3] Natl Ctr Genet Engn & Biotechnol, Thailand Sci Pk, Pathum Thani 12120, Thailand

[4] Thammasat Univ, Fac Dent, Pathum Thani 12120, Thailand

来源：

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL | 2016年 / 14卷

关键词：

Peptidomics; Cytotoxic peptides; Achatina fulica; Breast cancer; Bioinformatics prediction; Snail mucus; ANTIMICROBIAL PEPTIDE; ANTITUMOR; SERVER;

D O I：

10.1016/j.csbj.2015.11.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several reports have shown antimicrobial and anticancer activities of mucous glycoproteins extracted from the giant African snail Achatina fulica. Anticancer properties of the snail mucous peptides remain incompletely revealed. The aim of this study was to predict anticancer peptides from A. fulica mucus. Two of HPLC-separated mucous fractions (F2 and F5) showed in vitro cytotoxicity against the breast cancer cell line (MCF-7) and normal epithelium cell line (Vero). According to the mass spectrometric analysis, 404 and 424 peptides from the F2 and F5 fractions were identified. Our comprehensive bioinformatics workflow predicted 16 putative cationic and amphipathic anticancer peptides with diverse structures from these two peptidome data. These peptides would be promising molecules for new anti-breast cancer drug development. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of the Research Network of Computational and Structural Biotechnology.

引用

页码：49 / 57

页数：9

共 33 条

[1] Alarifi S, 2011, AFR J BIOTECHNOL, V10, P3872
[2] Assembly and annotation of a non-model gastropod (Nerita melanotragus) transcriptome: A comparison of de novo assemblers
Amin S.
Prentis P.J.
Gilding E.K.
Pavasovic A.
[J]. BMC Research Notes, 7 (1)
[3] Anticancer α-helical peptides and structure/function relationships underpinning their interactions with tumour cell membranes
Dennison, Sarah R.
Whittaker, Michelle
Harris, Frederick
Phoenix, David A.
[J]. CURRENT PROTEIN & PEPTIDE SCIENCE, 2006, 7 (06) : 487 - 499
[4] Freshney RI., 1994, Culture of animal cells : A manual of basic technique, V3rd, DOI DOI 10.1002/9780470649367
[5] In silico approaches for designing highly effective cell penetrating peptides
Gautam, Ankur
Chaudhary, Kumardeep
Kumar, Rahul
Sharma, Arun
Kapoor, Pallavi
Tyagi, Atul
Raghava, Gajendra P. S.
[J]. JOURNAL OF TRANSLATIONAL MEDICINE, 2013, 11
[6] Trends in breast cancer by race and ethnicity
Ghafoor, A
Jemal, A
Ward, E
Cokkinides, V
Smith, R
Thun, M
[J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2003, 53 (06) : 342 - 355
[7] Goldstein NS., 2011, Early Diagnosis and Treatment of Cancer Series: Breast Cancer, P55, DOI [DOI 10.1016/B978-1-4160-4932-6.50009-3, 10.1016/B978-1-4160-4932-6.50009-3]
[8] HANCOCK WS, 1984, HDB HPLC SEPARATION, V2
[9] HANCOCK WS, 1984, HDB HPLC SEPARATION, V1
[10] On the selectivity and efficacy of defense peptides with respect to cancer cells
Harris, Frederick
Dennison, Sarah R.
Singh, Jaipaul
Phoenix, David A.
[J]. MEDICINAL RESEARCH REVIEWS, 2013, 33 (01) : 190 - 234

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