Cloning and expression analysis of two novel PCTAIRE 3 transcripts from human brain

被引:11
作者
Herskovits, AZ
Davies, P
机构
[1] Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10461 USA
[2] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA
关键词
cell cycle; cdk-family; isoforms; kinase; genomic structure;
D O I
10.1016/j.gene.2003.12.011
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
PCTAIRE 3 is a member of the PCTAIRE subfamily of cdc2-related serine/threonine protein kinases. In the present study, cDNAs encoding two isoforms of PCTAIRE 3 have been cloned and the genomic organization of the human PCTAIRE 3 gene is reported. The gene spans 28.15 kb on chromosome 1q31-32 and contains 16 exons. The major transcript of PCTAIRE 3, designated PCTAIPE 3a, has an open reading frame that is 474 amino acids in length. Transcripts for PCTAIRE 3a were evident throughout the brain and in the majority of tissues analyzed. A second transcript containing an insert that adds 90 nucleotides to the third exon of the gene was also identified. This transcript, designated PCTAIRE 3b, encodes a polypeptide of 504 amino acids. Expression of PCTAIRE 3b was limited to several subcortical nuclei of the basal gangli and the spinal cord and substantial levels of this transcript were not evident outside of the central nervous system. Primary sequence comparisons between different cdc2-related serine/threonine protein kinases reveal that these proteins are most heterogeneous in their N-terminal domains and the PCTAIRE subfamily is further diversified by the presence of isoforms within this region. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:59 / 67
页数:9
相关论文
共 30 条
[1]  
Charrasse S, 1999, CELL GROWTH DIFFER, V10, P611
[2]   Pctaire1 interacts with p35 and is a novel substrate for Cdk5/p35 [J].
Cheng, K ;
Li, Z ;
Fu, WY ;
Wang, JH ;
Fu, AKY ;
Ip, NY .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (35) :31988-31993
[3]   Expression of mitochondrial genes and of the transcription factors involved in the biogenesis of mitochondria Tfam, NRF-1 and NRF-2, in rat liver, testis and brain [J].
Escrivá, H ;
Rodríguez-Peña, A ;
Vallejo, CG .
BIOCHIMIE, 1999, 81 (10) :965-971
[4]   Signaling complex organization by PDZ domain proteins [J].
Fan, JS ;
Zhang, MJ .
NEUROSIGNALS, 2002, 11 (06) :315-321
[5]   Expression of alternatively spliced PCTAIRE-1 mRNA in PC12 cells and neonatal rat brain [J].
Gao, CY ;
Chauthaiwale, VM ;
Rampalli, AM ;
Zelenka, PS .
GENE, 1996, 176 (1-2) :243-247
[6]  
Graeser R, 2002, J CELL SCI, V115, P3479
[7]   Alternative splicing of Drosophila calcium/calmodulin-dependent protein kinase II regulates substrate specificity and activation [J].
GuptaRoy, B ;
Marwaha, N ;
Pla, M ;
Wang, Z ;
Nelson, HB ;
Beckingham, K ;
Griffith, LC .
MOLECULAR BRAIN RESEARCH, 2000, 80 (01) :26-34
[8]   3 ADDITIONAL GENES REQUIRED FOR DEOXYRIBONUCLEIC ACID SYNTHESIS IN SACCHAROMYCES-CEREVISIAE [J].
HARTWELL, LH .
JOURNAL OF BACTERIOLOGY, 1973, 115 (03) :966-974
[9]   Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL [J].
Heinemeyer, T ;
Wingender, E ;
Reuter, I ;
Hermjakob, H ;
Kel, AE ;
Kel, OV ;
Ignatieva, EV ;
Ananko, EA ;
Podkolodnaya, OA ;
Kolpakov, FA ;
Podkolodny, NL ;
Kolchanov, NA .
NUCLEIC ACIDS RESEARCH, 1998, 26 (01) :362-367
[10]   Identification of tudor repeat associator with PCTAIRE 2 (Trap) - A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain [J].
Hirose, T ;
Kawabuchi, M ;
Tamaru, T ;
Okumura, N ;
Nagai, K ;
Okada, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (07) :2113-2121