Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi

被引：8

作者：

Ma, Xin-Ye ^{[1
]}

Yi, Gan-Jun ^{[2
]}

Huang, Xue-Lin ^{[1
]}

Zeng, Ji-Wu ^{[2
]}

机构：

[1] Zhongshan Sun Yat Sen Univ, Sch Life Sci, Key Lab Gene Engn, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[2] Guangdong Acad Agr Sci, Fruit Res Inst, Guangzhou 510640, Guangdong, Peoples R China

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2009年 / 31卷 / 02期

关键词：

Explant orientation; Friable calli; Globular embryos; Leaflet age; Leaflet position; Light regime; Lychee; ADVENTITIOUS SHOOT REGENERATION; SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; EXPLANTS; GENOTYPE; LEAVES;

D O I：

10.1007/s11738-008-0223-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This study reports a protocol for leaf callus induction and suspension culture establishment in lychee cv. Huaizhi. The results showed that 12-day-old leaf explants cultured under a photoperiod of 16/8 h with their adaxial side touching the medium were the optimum conditions for leaf callus induction. Globular embryos were formed when the induced calli were kept on the callus induction medium without 2,4-D for 24 weeks. Friable calli were induced after 2-3 subcultures at 4 weeks intervals on the Murashige and Skoog medium supplemented with 3 mg/L IAA and 2 mg/L BAP. Suspension culture was established when these friable calli were subcultured six times in liquid callus induction medium.

引用

页码：401 / 405

页数：5

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