U2504 Determines the Species Specificity of the A-Site Cleft Antibiotics: The Structures of Tiamulin, Homoharringtonine, and Bruceantin Bound to the Ribosome

被引：153

作者：

Guerel, Gueliz ^{[1
]}

Blaha, Gregor ^{[2
]}

Moore, Peter B. ^{[1
,2
]}

Steitz, Thomas A. ^{[1
,2
,3
]}

机构：

[1] Yale Univ, Dept Chem, New Haven, CT 06520 USA

[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[3] Howard Hughes Med Inst, New Haven, CT 06520 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2009年 / 389卷 / 01期

基金：

美国国家卫生研究院;

关键词：

antibiotic specificity; large ribosomal subunit; tiamulin; homoharringtonine; bruceantin; PEPTIDYL-TRANSFERASE CENTER; PROTEIN-SYNTHESIS; PLEUROMUTILIN DERIVATIVES; BINDING-SITE; TRANSFER-RNA; SUBUNIT; INHIBITION; LOCATION; MODE; CHLORAMPHENICOL;

D O I：

10.1016/j.jmb.2009.04.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structures have been obtained for the complexes that tiamulin, homoharringtonine, and bruceantin form with the large ribosomal subunit of Haloarcula marismortui at resolutions ranging from 2.65 to 3.2 angstrom. They show that all these inhibitors block protein synthesis by competing with the amino acid side chains of incoming aminoacyl-tRNAs for binding in the A-site cleft in the peptidyl-transferase center, which is universally conserved. In addition, these structures support the hypothesis that the species specificity exhibited by the A-site cleft inhibitors is determined by the interactions they make, or fail to make, with a single nucleotide, U2504 (Escherichia coli). In the ribosome, the position of U2504 is controlled by its interactions with neighboring nucleotides, whose identities vary among kingdoms. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：146 / 156

页数：11

共 51 条

[1] The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution [J].