Advanced time-correlated single photon counting technique for spectroscopy and imaging of biological systems

被引：2

作者：

Becker, Wolfgang ^{[1
]}

Bergmann, Axel ^{[1
]}

Kacprzak, Michal ^{[2
]}

Liebert, Adam ^{[2
]}

机构：

[1] Becker & Hickl GmbH, Nahmitzer Damm 30, D-12277 Berlin, Germany

[2] Inst Biocybermet & Biomed Engn, PL-02109 Warsaw, Poland

来源：

FOURTH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE, PTS 1 AND 2 | 2006年 / 6047卷

关键词：

TCSP; DOT; fluorescence; autofluorescence; FLIM; FRET; microscopy;

D O I：

10.1117/12.710872

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We present a multi-dimensional TCSPC technique that simultaneously records the photon distribution over the time in the fluorescence decay, the wavelength, and the coordinates of a two-dimensional scan or the time since the start of the experiment. We demonstrate the application of the technique to diffuse optical tomography, single-point autofluorescence measurements of skin, and multi-spectral autofluorescence lifetime imaging of tissue.

引用

页数：5

共 6 条

[1] Fluorescence lifetime imaging by time-correlated single-photon counting
Becker, W
Bergmann, A
Hink, MA
König, K
Benndorf, K
Biskup, C
[J]. MICROSCOPY RESEARCH AND TECHNIQUE, 2004, 63 (01) : 58 - 66
[2] Becker W., 2005, ADV TIME CORRELATED
[3] Simultaneous reconstruction of absorption and scattering images by multichannel measurement of purely temporal data
Hebden, JC
Schmidt, FEW
Fry, ME
Schweiger, M
Hillman, EMC
Delpy, DT
Arridge, SR
[J]. OPTICS LETTERS, 1999, 24 (08) : 534 - 536
[4] Lakowicz J.R., 2004, PRINCIPLES FLUORESCE, V3rd
[5] Time-resolved multidistance near-infrared spectroscopy of the adult head:: intracerebral and extracerebral absorption changes from moments of distribution of times of flight of photons
Liebert, A
Wabnitz, H
Steinbrink, J
Obrig, H
Möller, M
Macdonald, R
Villringer, A
Rinneberg, H
[J]. APPLIED OPTICS, 2004, 43 (15) : 3037 - 3047
[6] OConnor D., 2012, Time-correlated single-photon counting

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