Effects of chlorpromazine, pentoxifylline and dexamethasone on mRNA expression of lipopolysaccharide-induced inflammatory cytokines in bovine peripheral blood mononuclear cells

被引：16

作者：

Kiku, Y ^{[1
]}

Matsuzawa, H

Ohtsuka, H

Terasaki, N

Fukuda, S

Kon-Nai, S

Koiwa, M

Yokomizo, Y

Sato, H

Rosol, TJ

Okada, H

Yoshino, TO

机构：

[1] Rakuno Gakuen Univ, Sch Vet Med, Large Anim Clin Ctr, Dept Vet Pathol, Ebetsu, Hokkaido 0698501, Japan

[2] Rakuno Gakuen Univ, Sch Vet Med, Large Anim Clin Ctr, Dept Vet Internal Med, Ebetsu, Hokkaido 0698501, Japan

[3] Rakuno Gakuen Univ, Sch Vet Med, Large Anim Clin Ctr, Vet Teaching Hosp, Ebetsu, Hokkaido 0698501, Japan

[4] Kitasato Univ, Sch Vet Med, Dept Vet Internal Med, Towada, Aomori 0348628, Japan

[5] Hokkaido Univ, Infect Dis Lab, Grad Sch Vet Med, Dept Dis Control, Sapporo, Hokkaido 0600818, Japan

[6] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan

[7] Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2002年 / 64卷 / 08期

关键词：

bovine; cytokine; lipopolysaccharide;

D O I：

10.1292/jvms.64.723

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The effects of chlorpromazine (CPZ), pentoxifylline (PTX) and dexamethasone (DEX) on mRNA expression of lipopolysaccharide (LPS)-induced proinflammatory cytokines were examined in bovine peripheral blood mononuclear cells (PBMCs) in vitro. The expression of inflammatory cytokine mRNAs was analyzed by RT-PCR and Southern blot hybridization in bovine PBMCs. CPZ and DEX decreased the expression of cytokine mRNA (such as interleukin-1beta and tumor necrosis factor-a) after stimulation with LPS in a dose-dependent manner. However, pretreatment with PTX had no inhibitory effect on the mRNA expression of proinflammatory cytokines. These results indicated that pretreatment with CPZ and DEX might be effective to reduce the production of LPS-induced inflammatory cytokines in bovine PBMCs in vitro.

引用

页码：723 / 726

页数：4

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