The Equine Movement Disorder "Shivers" Is Associated With Selective Cerebellar Purkinje Cell Axonal Degeneration

被引:21
作者
Valberg, S. J. [1 ]
Lewis, S. S. [1 ]
Shivers, J. L. [2 ]
Barnes, N. E. [1 ]
Konczak, J. [3 ]
Draper, A. C. E. [1 ]
Armien, A. G. [1 ,2 ]
机构
[1] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA
[2] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA
[3] Univ Minnesota, Sch Kinesiol, St Paul, MN 55108 USA
关键词
axonal degeneration; cerebellum; horse; myelin decompaction; myoclonus; Purkinje cell; shivering; POLYSACCHARIDE STORAGE MYOPATHY; DENTATE NUCLEUS; DRAFT HORSES; MOUSE; WALKING; PATTERN;
D O I
10.1177/0300985815571668
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
"Shivers" is a progressive equine movement disorder of unknown etiology. Clinically, horses with shivers show difficulty walking backward, assume hyperflexed limb postures, and have hind limb tremors during backward movement that resembles shivering. At least initially, forward movements are normal. Given that neither the neurophysiologic nor the pathologic mechanisms of the disease is known, nor has a neuroanatomic locus been identified, we undertook a detailed neuroanatomic and neuropathologic analysis of the complete sensorimotor system in horses with shivers and clinically normal control horses. No abnormalities were identified in the examined hind limb and forelimb skeletal muscles nor the associated peripheral nerves. Eosinophilic segmented axonal spheroids were a common lesion. Calretinin-positive axonal spheroids were present in many regions of the central nervous system, particularly the nucleus cuneatus lateralis; however, their numbers did not differ significantly from those of control horses. When compared to controls, calretinin-negative, calbindin-positive, and glutamic acid decarboxylase-positive spheroids were increased 80-fold in Purkinje cell axons within the deep cerebellar nuclei of horses with shivers. Unusual lamellar or membranous structures resembling marked myelin decompaction were present between myelin sheaths of presumed Purkinje cell axons in the deep cerebellar nuclei of shivers but not control horses. The immunohistochemical and ultrastructural characteristics of the lesions combined with their functional neuroanatomic distribution indicate, for the first time, that shivers is characterized by end-terminal neuroaxonal degeneration in the deep cerebellar nuclei, which results in context-specific hypermetria and myoclonus.
引用
收藏
页码:1087 / 1098
页数:12
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