Automated data collection in single particle electron microscopy

被引：41

作者：

Tan, Yong Zi ^{[1
,2
,3
]}

Cheng, Anchi ^{[1
,2
]}

Potter, Clinton S. ^{[1
,2
,3
]}

Carragher, Bridget ^{[1
,2
,3
]}

机构：

[1] New York Struct Biol Ctr, Natl Resource Automated Mol Microscopy, New York, NY 10027 USA

[2] New York Struct Biol Ctr, Simons Electron Microscopy Ctr, New York, NY 10027 USA

[3] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

来源：

MICROSCOPY | 2016年 / 65卷 / 01期

基金：

美国国家卫生研究院;

关键词：

single particle electron microscopy; data collection; automation; target acquisition; software packages; high throughput; COMA-FREE ALIGNMENT; CRYO-EM; CRYOELECTRON MICROSCOPY; DATA-ACQUISITION; CCD CAMERAS; ANGSTROM RESOLUTION; 3-FOLD ASTIGMATISM; STRUCTURAL BIOLOGY; 2D CRYSTALLIZATION; SOFTWARE PLATFORM;

D O I：

10.1093/jmicro/dfv369

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Automated data collection is an integral part of modern workflows in single particle electron microscopy (EM) research. This review surveys the software packages available for automated single particle EM data collection. The degree of automation at each stage of data collection is evaluated, and the capabilities of the software packages are described. Finally, future trends in automation are discussed.

引用

页码：43 / 56

页数：14