Dysregulation of GdA Expression in Endometrium of Women With Endometriosis: Implication for Endometrial Receptivity

被引：32

作者：

Focarelli, Riccardo ^{[1
]}

Luddi, Alice ^{[2
,3
]}

De Leo, Vincenzo ^{[2
,3
]}

Capaldo, Angela ^{[2
,3
]}

Stendardi, Anita ^{[2
]}

Pavone, Valentina ^{[1
]}

Benincasa, Linda ^{[1
]}

Belmonte, Giuseppe ^{[2
]}

Petraglia, Felice ^{[2
,3
]}

Piomboni, Paola ^{[2
,3
]}

机构：

[1] Univ Siena, Dept Life Sci, Siena, Italy

[2] Univ Siena, Dept Mol & Dev Med, Via Aldo Moro 2, I-53100 Siena, Italy

[3] S Maria Scotte Hosp, Ctr Diag & Treatment Couple Steril, Siena, Italy

来源：

REPRODUCTIVE SCIENCES | 2018年 / 25卷 / 04期

关键词：

endometriosis; glycodelin-A; human endometrium; menstrual cycle; 2-D electrophoresis; GLYCODELIN-A; 2-DIMENSIONAL ELECTROPHORESIS; OVARIAN ENDOMETRIOSIS; PLACENTAL PROTEIN-14; REDUCED EXPRESSION; PERITONEAL-FLUID; MENSTRUAL-CYCLE; EARLY-PREGNANCY; IMPLANTATION; BIOMARKERS;

D O I：

10.1177/1933719117718276

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Glycodelin-A (GdA) has been proposed to represent a potential biomarker of endometrial function, but little is known about its expression during the different phases of the menstrual cycle and under pathological conditions. In the light of its potential importance also in embryo implantation, we aimed to evaluate the expression profile of GdA as well as the presence of different glycosylated glycoforms and the immunolocalization in endometrial tissue from women with endometriosis and in women with proven fertility, at different times during the menstrual cycle. Our results showed that GdA is synthesized by endometrial epithelial and stromal cells, both in healthy endometrium and eutopic endometrium from women with endometriosis, with a profile including several glycosylated glycoforms, differentially expressed in each phase of the menstrual cycle. During the secretory phase, a significant increase in GdA protein expression, with a different glycoforms profile, was observed in endometriotic eutopic endometrium. Protein localization in eutopic endometrial tissue resulted significantly different in comparison with endometrium from women with proven fertility. This study indicate that GdA is a complex glycoprotein including up to 6 different glycoforms specifically expressed during the different phase of the menstrual cycle; in pathologic conditions such as endometriosis, the expression profile is altered possibly related to the impaired endometrial receptivity.

引用

页码：579 / 586

页数：8

共 34 条

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