Photophysical study of Thioflavin T as fluorescence marker of amyloid fibrils

被引：95

作者：

Freire, Sonia ^{[1
]}

de Araujo, Marcus H. ^{[1
]}

Al-Soufi, Wajih ^{[1
]}

Novo, Mercedes ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Ciencias, Dept Quim Fis, E-27002 Lugo, Spain

来源：

DYES AND PIGMENTS | 2014年 / 110卷

关键词：

Thioflavin T; Amyloid fibril; Fluorescent marker; Photophysical properties; Molecular rotor; TICT; ABSORPTION-SPECTRA; CHARGE-TRANSFER; EXCITED-STATE; CONGO RED; IN-VITRO; BETA; BINDING; PROTEIN; DYE; OLIGOMERS;

D O I：

10.1016/j.dyepig.2014.05.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Thioflavin T is a highly sensitive fluorescent marker of amyloid fibrils that has been widely used for in vitro biomedical assays. However, neither its complex photophysical behavior nor its binding mode to amyloid fibrils are still well understood. We present a detailed analysis of the photophysical properties of Thioflavin T in various media, including solvents and solvent mixtures of different viscosities as well as fibrillar and globular proteins. We propose a model that explains the strong wavelength dependency of the Thioflavin T fluorescence and the large fluorescence enhancement in certain environments. We determine the binding affinities and the fluorescence properties of Thioflavin T bound to amyloid-beta (1-42) fibrils and to bovine serum albumin and discuss the sensitivity and the specificity of this probe to amyloid aggregates. These results allow us to assess the suitability of Thioflavin T for quantitative determinations in biomedical studies. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：97 / 105

页数：9

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