Monosize magnetic hydrophobic beads for lysozyme purification under magnetic field

被引：27

作者：

Altintas, Evrim Banu ^{[1
]}

Denizli, Adil ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2009年 / 29卷 / 05期

关键词：

Protein purification; Hydrophobic interaction chromatography; Lysozyme; Magnetic beads; EXPANDED BED ADSORPTION; IMMUNOGLOBULIN PRODUCTION; PROTEIN-PURIFICATION; AFFINITY; ADSORBENT; SUPPORT; RESIN;

D O I：

10.1016/j.msec.2008.12.028

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Monosize and magnetic poly(glycidyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPGMATrp] beads (1.6 mu m in diameter) were used for hydrophobic affinity capture of lysozyme from chicken egg-white. N-methacryloyl-(L)-tryptophan (MATrp), which gives hydrophobicity to the resulting polymer, was synthesized by reacting methacryloyl chloride and L-tryptophan methyl ester then characterized by Nuclear Magnetic Resonance (NMR). mPGMATrp beads were produced by dispersion polymerization in the presence of magnetite nano-powder. mPGMATrp beads were characterized by means of swelling studies, elemental analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Lysozyme adsorption experiments were performed under different experimental conditions (i.e., lysozyme concentration. temperature. and ionic strength) in magnetically stabilized fluidized bed system, (MSFB). Maximum adsorption capacity was 263.9 mg/g. It was observed that mPGMATrp beads call be used without significant loss in lysozyme adsorption capacity after 25 adsorption-elution Cycle. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1627 / 1634

页数：8

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