Monosize magnetic hydrophobic beads for lysozyme purification under magnetic field

被引：27

作者：

Altintas, Evrim Banu ^{[1
]}

Denizli, Adil ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2009年 / 29卷 / 05期

关键词：

Protein purification; Hydrophobic interaction chromatography; Lysozyme; Magnetic beads; EXPANDED BED ADSORPTION; IMMUNOGLOBULIN PRODUCTION; PROTEIN-PURIFICATION; AFFINITY; ADSORBENT; SUPPORT; RESIN;

D O I：

10.1016/j.msec.2008.12.028

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Monosize and magnetic poly(glycidyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPGMATrp] beads (1.6 mu m in diameter) were used for hydrophobic affinity capture of lysozyme from chicken egg-white. N-methacryloyl-(L)-tryptophan (MATrp), which gives hydrophobicity to the resulting polymer, was synthesized by reacting methacryloyl chloride and L-tryptophan methyl ester then characterized by Nuclear Magnetic Resonance (NMR). mPGMATrp beads were produced by dispersion polymerization in the presence of magnetite nano-powder. mPGMATrp beads were characterized by means of swelling studies, elemental analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Lysozyme adsorption experiments were performed under different experimental conditions (i.e., lysozyme concentration. temperature. and ionic strength) in magnetically stabilized fluidized bed system, (MSFB). Maximum adsorption capacity was 263.9 mg/g. It was observed that mPGMATrp beads call be used without significant loss in lysozyme adsorption capacity after 25 adsorption-elution Cycle. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1627 / 1634

页数：8

共 30 条

[1] Cu(II)-incorporated histidine-containing, magnetic-metal-complexing beads as specific sorbents for the metal chelate affinity of albumin [J].

Akgöl, S ;

Türkmen, D ;

Denizli, A .

JOURNAL OF APPLIED POLYMER SCIENCE, 2004, 93 (06) :2669-2677

[2] Synthesis and characterization of monosize magnetic poly(glycidyl methacrylate) beads [J].

Altintas, Evrim Banu ;

Uzun, Lokman ;

Denizli, Adil .

CHINA PARTICUOLOGY, 2007, 5 (1-2) :174-179

[3] Use of magnetic poly(glycidyl methacrylate) monosize beads for the purification of lysozyme in batch system [J].

Altintas, Evrim Banu ;

Tuezmen, Nalan ;

Candan, Nilgun ;

Denizli, Adil .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 853 (1-2) :105-113

[4] Lysozyme purification with dye-affinity beads under magnetic field [J].

Basar, Nilguen ;

Uzun, Lokman ;

Guener, Ali ;

Denizli, Adil .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2007, 41 (03) :234-242

[5] Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels [J].

Bereli, Nilay ;

Andac, Muege ;

Baydemir, Goezde ;

Say, Ridvan ;

Galaev, Igor Yu ;

Denizli, Adil .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1190 (1-2) :18-26

[6] Antibody separation by hydrophobic charge induction chromatography [J].

Boschetti, E .

TRENDS IN BIOTECHNOLOGY, 2002, 20 (08) :333-337

[7]

Chang YK, 1996, BIOTECHNOL BIOENG, V49, P512, DOI 10.1002/(SICI)1097-0290(19960305)49:5<512::AID-BIT4>3.3.CO

[8]

2-X

[9] Expanded bed adsorption of protein with DEAE Spherodex M [J].

Chen, WD ;

Tong, XD ;

Dong, XY ;

Sun, Y .

BIOTECHNOLOGY PROGRESS, 2003, 19 (03) :880-886

[10] The hydrophobic interactions of the ion-exchanger resin ligands with proteins at high salt concentrations by adsorption isotherms and isothermal titration calorimetry [J].

Chen, Wen-Yih ;

Liu, Zhen-Chia ;

Lin, Po-Hsun ;

Fang, Chiung-I ;

Yamamoto, Shuichi .

SEPARATION AND PURIFICATION TECHNOLOGY, 2007, 54 (02) :212-219

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