Comparison of stirred tank and airlift bioreactors in the production of polygalacturonases by Aspergillus oryzae

被引：43

作者：

Fontana, Roselei Claudete ^{[1
]}

Polidoro, Tomas Augusto ^{[1
]}

da Silveira, Mauricio Moura ^{[1
]}

机构：

[1] Univ Caxias Sul, Inst Biotecnol, BR-95001970 Caxias Do Sul, RS, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 19期

关键词：

Aspergillus oryzae; Endo-polygalacturonase; Exo-polygalacturonase; Stirred tank bioreactor; Airlift bioreactor; SOLID-STATE FERMENTATION; SUBMERGED CULTURES; STATISTICAL DESIGN; PROTEIN-PRODUCTION; ALPHA-AMYLASE; NIGER; OPTIMIZATION; ENZYMES; FUNGUS; PURIFICATION;

D O I：

10.1016/j.biortech.2008.11.062

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The production of endo and exo-polygalacturonase (PG) by Aspergillus oryzae IPT 301 was studied in a stirred tank bioreactor (STR) and an internal circulation airlift bioreactor. Using a factorial experimental design, a soluble Culture medium was defined which allowed the production of exo- and endo-PG comparable to that obtained in a medium containing suspended wheat bran. The soluble medium was used in tests to compare the production of these enzymes in the STIR and airlift bioreactor. in these tests, after 96 h, maximum enzymatic activity values achieved for exo- and endo-PG were 65.2 units (U) per mL and 91.3 U mL(-1), in the STR, with similar activity Values of 60.6 U mL(-1) and 86.2 U mL(-1), respectively, being achieved in the airlift bioreactor. The airlift bioreactor also showed satisfactory results regarding the oxygen transfer rate in this process, indicating its potential to be used in an eventual larger scale production of exo- and endo-PG. with lower costs for both installation and operation. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4493 / 4498

页数：6

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