Quantitative real-time polymerase chain reaction is an alternative method for the detection of HER-2 amplification in formalin-fixed paraffin-embedded breast cancer samples

被引：0
作者：
Pu, Tianjie [1 ,2 ]
Guo, Peng [1 ]
Qiu, Yan [1 ,2 ]
Chen, Shinan [1 ]
Yang, Libo [1 ,2 ]
Sun, Linyong [2 ]
Ye, Feng [1 ]
Bu, Hong [1 ,2 ]
机构：
[1] Sichuan Univ, West China Hosp, Pathol Lab, Chengdu 610041, Sichuan, Peoples R China
[2] Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 09期
基金：
中国国家自然科学基金;
关键词：
HER-2 copy number variation; breast neoplasm; quantitative real-time polymerase chain reaction; IHC; FISH; delayed formalin-fixed; IN-SITU HYBRIDIZATION; GENE AMPLIFICATION; COPY-NUMBER; ADJUVANT CHEMOTHERAPY; PROTEIN EXPRESSION; AMERICAN-SOCIETY; POOR-PROGNOSIS; IMMUNOHISTOCHEMISTRY; RECEPTOR; PCR;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common methods that are used to quantify HER-2 gene and protein levels, respectively, in human breast cancer. However, due to bad sample quality, some samples are unable to be subjected to a FISH assay. We evaluated 71 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by quantitative real-time polymerase chain reaction (qPCR), IHC, and FISH. We also performed qPCR and FISH assays on delayed formalin-fixed (DDF) samples. The qPCR results were in complete concordance with the results of IHC and FISH. In regards to the DDF samples, the HER-2 fluorescent signal seemed decayed compared with that of the DDF samples after 1 h. However, the qPCR method still works well up to 12 hours. Our results indicated that qPCR was obviously superior to FISH in cases that were not fixed in a reasonable amount of time. However, qPCR can be an alternative method by which to perform HER2 amplification assays in breast cancer.
引用
收藏
页码：10565 / 10574
页数：10
相关论文
共 41 条
  • [1] neu/erbB-2 amplification identifies a poor-prognosis group of women with node-negative breast cancer
    Andrulis, IL
    Bull, SB
    Blackstein, ME
    Sutherland, D
    Mak, C
    Sidlofsky, S
    Pritzker, KPH
    Hartwick, RW
    Hanna, W
    Lickley, L
    Wilkinson, R
    Qizilbash, A
    Ambus, U
    Lipa, M
    Weizel, H
    Katz, A
    Baida, M
    Mariz, S
    Stoik, G
    Dacamara, P
    Strongitharm, D
    Geddie, W
    McCready, D
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (04) : 1340 - 1349
  • [2] Detection and quantitation of HER-2 gene amplification and protein expression in breast carcinoma
    Bofin, AM
    Ytterhus, B
    Martin, C
    O'Leary, JJ
    Hagmar, BM
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2004, 122 (01) : 110 - 119
  • [3] Bunn PA, 2001, CLIN CANCER RES, V7, P3239
  • [4] TYROSINE KINASE RECEPTOR WITH EXTENSIVE HOMOLOGY TO EGF RECEPTOR SHARES CHROMOSOMAL LOCATION WITH NEU ONCOGENE
    COUSSENS, L
    YANGFENG, TL
    LIAO, YC
    CHEN, E
    GRAY, A
    MCGRATH, J
    SEEBURG, PH
    LIBERMANN, TA
    SCHLESSINGER, J
    FRANCKE, U
    LEVINSON, A
    ULLRICH, A
    [J]. SCIENCE, 1985, 230 (4730) : 1132 - 1139
  • [5] Image analysis as an adjunct to manual HER-2 immunohistochemical review: a diagnostic tool to standardize interpretation
    Dobson, Lynne
    Conway, Catherine
    Hanley, Alan
    Johnson, Alex
    Costello, Sean
    O'Grady, Anthony
    Connolly, Yvonne
    Magee, Hilary
    O'Shea, Daniel
    Jeffers, Michael
    Kay, Elaine
    [J]. HISTOPATHOLOGY, 2010, 57 (01) : 27 - 38
  • [6] An Alternative and Reliable Real-time Quantitative PCR Method to Determine HER2/neu Amplification in Breast Cancer
    Egervari, Kristof
    Toth, Judit
    Nemes, Zoltan
    Szollosi, Zoltan
    [J]. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2009, 17 (03) : 247 - 254
  • [7] Evaluation of HER-2/NEU protein expression in breast cancer by immunohistochemistry:: an interlaboratory study assessing the reproducibility of HER-2/NEU testing
    Gancberg, D
    Järvinen, T
    di Leo, A
    Rouas, G
    Cardoso, F
    Paesmans, M
    Verhest, A
    Piccart, MJ
    Isola, J
    Larsimont, D
    [J]. BREAST CANCER RESEARCH AND TREATMENT, 2002, 74 (02) : 113 - 120
  • [8] Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma -: An alternative method for HER-2/neu analysis
    Gjerdrum, LM
    Sorensen, BS
    Kjeldsen, E
    Sorensen, FB
    Nexo, E
    Hamilton-Dutoit, S
    [J]. JOURNAL OF MOLECULAR DIAGNOSTICS, 2004, 6 (01) : 42 - 51
  • [9] Goud KI, 2012, INDIAN J MED RES, V135, P312
  • [10] Assessment of the HER2 status in breast cancer by fluorescence in situ hybridization:: a technical review with interpretive guidelines
    Hicks, DG
    Tubbs, RR
    [J]. HUMAN PATHOLOGY, 2005, 36 (03) : 250 - 261
12345