Photothermal spectral-domain optical coherence reflectometry for direct measurement of hemoglobin concentration of erythrocytes

被引：14

作者：

Yim, Jinyeong ^{[1
]}

Kim, Hun ^{[1
]}

Ryu, Suho ^{[1
]}

Song, Sungwook ^{[2
]}

Kim, Hyun Ok ^{[2
]}

Hyun, Kyung-A ^{[1
]}

Jung, Hyo-Il ^{[1
]}

Joo, Chulmin ^{[1
]}

机构：

[1] Yonsei Univ, Sch Mech Engn, Seoul 120749, South Korea

[2] Yonsei Univ, Coll Med, Dept Lab Med, Seoul 120749, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 57卷

基金：

新加坡国家研究基金会;

关键词：

Photothermal effect; Hemoglobin concentration; Erythrocytes; Low coherence interferometry; Refractive index; LIGHT-SCATTERING; CELLS; TOMOGRAPHY; ANEMIA; SYSTEM; VOLUME;

D O I：

10.1016/j.bios.2014.01.052

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel optical detection method for hemoglobin concentration is described. The hemoglobin molecules consisting mainly of iron generate heat upon their absorption of light energy at 532 nm, which subsequently changes the refractive index of the blood. We exploit this photothermal effect to determine the hemoglobin concentration of erythrocytes without any preprocessing of blood. Highly sensitive measurement of refractive index alteration of blood samples is enabled by a spectral-domain low coherence reflectometric sensor with subnanometer-level optical path-length sensitivity. The performance and validity of the sensor are presented by comparing the measured results against the reference data acquired from an automatic hematology analyzer. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：59 / 64

页数：6

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