Characterization of an antihypertensive peptide from an Alfalfa white protein hydrolysate produced by a continuous enzymatic membrane reactor

被引：61

作者：

Kapel, Romain ^{[1
]}

Rahhou, Elhassan ^{[1
]}

Lecouturier, Didier ^{[1
]}

Guillochon, Didier ^{[1
]}

Dhulster, Pascal ^{[1
]}

机构：

[1] IUT, Lab Technol Subst Nat, F-59655 Villeneuve, France

来源：

PROCESS BIOCHEMISTRY | 2006年 / 41卷 / 09期

关键词：

Alfalfa white proteins; RuBisCO; continuous membrane reactor; enzymatic proteolysis; ACE inhibitory peptides; antihypertensive peptides;

D O I：

10.1016/j.procbio.2006.04.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An industrial Alfalfa white protein concentrate hydrolysed at pilot plant scale by Delvolase (R) in a continuous enzymatic membrane reactor leads to a hydrolysate exhibiting a high ACE inhibition activity (8.8 mu g/ml). This hydrolysate, fractionated by two chromatography steps (exclusion size fast protein liquid chromatography and reversed-phase high performance liquid chromatography) revealed that around 15% of the total ACE inhibition activity is due to a single ACE inhibitory peptide. This peptide identified by electrospray mass spectrometry as VW, a potent antihypertensive peptide in vivo, contained four times of that in the main Alfalfa white protein (RuBisCO). (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1961 / 1966

页数：6

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