Metabolic engineering of Escherichia coli for production of fatty acid short-chain esters through combination of the fatty acid and 2-keto acid pathways

被引：52

作者：

Guo, Daoyi ^{[1
,2
,3
]}

Zhu, Jing ^{[1
,2
]}

Deng, Zixin ^{[1
,2
,3
]}

Liu, Tiangang ^{[1
,2
,3
]}

机构：

[1] Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China

[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China

[3] Wuhan Inst Biotechnol, Hubei Engn Lab Synthet Microbiol, Wuhan 430075, Peoples R China

来源：

METABOLIC ENGINEERING | 2014年 / 22卷

基金：

中国国家自然科学基金;

关键词：

Fatty acid short-chain esters; Short-chain alcohols; Acyltransferase; 2-keto acid decarboxylase; Alcohol dehydrogenase; WAX ESTER; DIACYLGLYCEROL ACYLTRANSFERASE; SACCHAROMYCES-CEREVISIAE; BIOSYNTHESIS; OVERPRODUCTION; EXPRESSION; CHEMICALS; SYNTHASE; STRAIN; FUELS;

D O I：

10.1016/j.ymben.2014.01.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fatty acid short-chain esters (FASEs) are biodiesels that are renewable, nontoxic, and biodegradable biofuels. A novel approach for the biosynthesis of FASEs has been developed using metabolically-engineered E. colt through combination of the fatty acid and 2-keto acid pathways. Several genetic engineering strategies were also developed to increase fatty acyl-CoA availability to improve FASEs production. Fed-batch cultivation of the engineered E. coli resulted in a titer of 1008 mg/L FASEs. Since the fatty acid and 2-keto acid pathways are native microbial synthesis pathways, this strategy can be implemented in a variety of microorganisms to produce various FASEs from cheap and readily-available, renewable, raw materials such as sugars and cellulose in the future. (C) 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

引用

页码：69 / 75

页数：7

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