Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

被引：7

作者：

Coelho Trevisan, Daliah Alves ^{[1
]}

Zanetti Campanerut-Sa, Paula Aline ^{[1
]}

da Silva, Alex Fiori ^{[2
]}

Pereira Batista, Andreia Farias ^{[1
]}

Vicente Seixas, Flavio Augusto ^{[3
]}

Peralta, Rosane Marina ^{[4
]}

Babeto De Sa-Nakanishi, Anacharis ^{[4
]}

de Abreu Filho, Benicio Alves ^{[5
]}

Machinski Junior, Miguel ^{[5
]}

Graton Mikcha, Jane Martha ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Clin Anal & Biomed, Maringa, Parana, Brazil

[2] Univ Estado Minas Gerais, Dept Biol Sci, Ituiutaba, Brazil

[3] Univ Estadual Maringa, Dept Technol, Umuarama, Brazil

[4] Univ Estadual Maringa, Dept Biochem, Maringa, Parana, Brazil

[5] Univ Estadual Maringa, Dept Basic Hlth Sci, Maringa, Parana, Brazil

来源：

JOURNAL OF APPLIED BIOMEDICINE | 2020年 / 18卷 / 04期

关键词：

Carvacrol; Biofilms; Proteome; Salmonella Typhimurium; ESCHERICHIA-COLI O157-H7; SUPEROXIDE-DISMUTASE; ESSENTIAL OILS; EXPRESSION; PROTEINS; DSBA; VIRULENCE; PATTERNS; PROFILE; GENES;

D O I：

10.32725/jab.2020.014

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.

引用

页码：106 / 114

页数：9

共 54 条

[51]

Wagon S, 1999, MATH ACTION PROBLEM

[52] Antimicrobial Activity of Naturally Occurring Phenols and Derivatives Against Biofilm and Planktonic Bacteria [J].

Walsh, Danica J. ;

Livinghouse, Tom ;

Goeres, Darla M. ;

Mettler, Madelyn ;

Stewart, Philip S. .

FRONTIERS IN CHEMISTRY, 2019, 7

[53] Removal of Salmonella biofilm formed under meat processing environment by surfactant in combination with bio-enzyme [J].

Wang, Huhu ;

Wang, Huawei ;

Xing, Tong ;

Wu, Na ;

Xu, Xinglian ;

Zhou, Guanghong .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2016, 66 :298-304

[54]

World Health Organization [WHO], 2018, SALM

← 1 2 3 4 5 6 →