Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

被引：7
作者：
Coelho Trevisan, Daliah Alves [1 ]
Zanetti Campanerut-Sa, Paula Aline [1 ]
da Silva, Alex Fiori [2 ]
Pereira Batista, Andreia Farias [1 ]
Vicente Seixas, Flavio Augusto [3 ]
Peralta, Rosane Marina [4 ]
Babeto De Sa-Nakanishi, Anacharis [4 ]
de Abreu Filho, Benicio Alves [5 ]
Machinski Junior, Miguel [5 ]
Graton Mikcha, Jane Martha [1 ]
机构：
[1] Univ Estadual Maringa, Dept Clin Anal & Biomed, Maringa, Parana, Brazil
[2] Univ Estado Minas Gerais, Dept Biol Sci, Ituiutaba, Brazil
[3] Univ Estadual Maringa, Dept Technol, Umuarama, Brazil
[4] Univ Estadual Maringa, Dept Biochem, Maringa, Parana, Brazil
[5] Univ Estadual Maringa, Dept Basic Hlth Sci, Maringa, Parana, Brazil
来源：
JOURNAL OF APPLIED BIOMEDICINE | 2020年 / 18卷 / 04期
关键词：
Carvacrol; Biofilms; Proteome; Salmonella Typhimurium; ESCHERICHIA-COLI O157-H7; SUPEROXIDE-DISMUTASE; ESSENTIAL OILS; EXPRESSION; PROTEINS; DSBA; VIRULENCE; PATTERNS; PROFILE; GENES;
D O I：
10.32725/jab.2020.014
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.
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收藏
页码：106 / 114
页数：9
相关论文
共 54 条
  • [1] Effect of carvacrol and thymol on Salmonella spp. biofilms on polypropylene
    Amaral, Viviane C. S.
    Santos, Patricia R.
    da Silva, Alex Fiori
    dos Santos, Adriele Rodrigues
    Machinski, Miguel, Jr.
    Graton Mikcha, Jane M.
    [J]. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2015, 50 (12) : 2639 - 2643
  • [2] Modulation of Biofilm-Formation in Salmonella enterica Serovar Typhimurium by the Periplasmic DsbA/DsbB Oxidoreductase System Requires the GGDEF-EAL Domain Protein STM3615
    Anwar, Naeem
    Rouf, Syed Fazle
    Romling, Ute
    Rhen, Mikael
    [J]. PLOS ONE, 2014, 9 (08):
  • [3] Novel Processed Form of Syndecan-1 Shed from SCC-9 Cells Plays a Role in Cell Migration
    Aragao, Annelize Z. B.
    Belloni, Marilia
    Simabuco, Fernando M.
    Zanetti, Mariana R.
    Yokoo, Sami
    Domingues, Romenia R.
    Kawahara, Rebeca
    Pauletti, Bianca A.
    Goncalves, Anderson
    Agostini, Michelle
    Graner, Edgard
    Coletta, Ricardo D.
    Fox, Jay W.
    Paes Leme, Adriana F.
    [J]. PLOS ONE, 2012, 7 (08):
  • [4] The Stringent Response Regulator DksA Is Required for Salmonella enterica Serovar Typhimurium Growth in Minimal Medium, Motility, Biofilm Formation, and Intestinal Colonization
    Azriel, Shalhevet
    Goren, Alina
    Rahav, Galia
    Gal-Mor, Ohad
    [J]. INFECTION AND IMMUNITY, 2016, 84 (01) : 375 - 384
  • [5] New Perspectives on the Use of Phytochemicals as an Emergent Strategy to Control Bacterial Infections Including Biofilms
    Borges, Anabela
    Abreu, Ana Cristina
    Dias, Carla
    Saavedra, Maria Jose
    Borges, Fernanda
    Simoes, Manuel
    [J]. MOLECULES, 2016, 21 (07):
  • [6] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [7] Biofilm-associated persistence of food-borne pathogens
    Bridier, A.
    Sanchez-Vizuete, P.
    Guilbaud, M.
    Piard, J. -C.
    Naitali, M.
    Briandet, R.
    [J]. FOOD MICROBIOLOGY, 2015, 45 : 167 - 178
  • [8] Essential oils: their antibacterial properties and potential applications in foods - a review
    Burt, S
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 94 (03) : 223 - 253
  • [9] The Natural Antimicrobial Carvacrol Inhibits Quorum Sensing in Chromobacterium violaceum and Reduces Bacterial Biofilm Formation at Sub-Lethal Concentrations
    Burt, Sara A.
    Ojo-Fakunle, Victoria T. A.
    Woertman, Jenifer
    Veldhuizen, Edwin J. A.
    [J]. PLOS ONE, 2014, 9 (04):
  • [10] Biofilm Formation in Food Processing Environments is Still Poorly Understood and Controlled
    Cappitelli, F.
    Polo, A.
    Villa, F.
    [J]. FOOD ENGINEERING REVIEWS, 2014, 6 (1-2) : 29 - 42
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