Assessing blood coagulation status with laser speckle rheology

被引：62

作者：

Tripathi, Markandey M. ^{[1
]}

Hajjarian, Zeinab ^{[1
]}

Van Cott, Elizabeth M. ^{[2
]}

Nadkarni, Seemantini K. ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA 02114 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Pathol, Boston, MA 02144 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 03期

关键词：

DIFFUSING-WAVE-SPECTROSCOPY; SURFACE-PLASMON RESONANCE; WHOLE-BLOOD; COMPLEX FLUIDS; ATHEROSCLEROTIC PLAQUES; VISCOELASTIC PROPERTIES; MONITORING COAGULATION; MECHANICAL-PROPERTIES; TISSUE; THROMBOELASTOGRAPHY;

D O I：

10.1364/BOE.5.000817

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed and investigated a novel optical approach, Laser Speckle Rheology (LSR), to evaluate a patient's coagulation status by measuring the viscoelastic properties of blood during coagulation. In LSR, a blood sample is illuminated with laser light and temporal speckle intensity fluctuations are measured using a high-speed CMOS camera. During blood coagulation, changes in the viscoelastic properties of the clot restrict Brownian displacements of light scattering centers within the sample, altering the rate of speckle intensity fluctuations. As a result, blood coagulation status can be measured by relating the time scale of speckle intensity fluctuations with clinically relevant coagulation metrics including clotting time and fibrinogen content. Our results report a close correlation between coagulation metrics measured using LSR and conventional coagulation results of activated partial thromboplastin time, prothrombin time and functional fibrinogen levels, creating the unique opportunity to evaluate a patient's coagulation status in real-time at the point of care. (C) 2014 Optical Society of America

引用

页码：817 / 831

页数：15

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