Hemagglutinin-Mediated Membrane Fusion: A Biophysical Perspective

被引:50
作者
Boonstra, Sander [1 ]
Blijleven, Jelle S. [1 ]
Roos, Wouter H. [1 ]
Onck, Patrick R. [1 ]
van der Giessen, Erik [1 ]
van Oijen, Antoine M. [2 ]
机构
[1] Univ Groningen, Zernike Inst Adv Mat, NL-9747 AG Groningen, Netherlands
[2] Univ Wollongong, Sch Chem, Fac Sci Med & Hlth, Wollongong, NSW 2522, Australia
来源
ANNUAL REVIEW OF BIOPHYSICS, VOL 47 | 2018年 / 47卷
基金
欧洲研究理事会; 澳大利亚研究理事会;
关键词
hemagglutinin; membrane fusion; viral fusion; influenza cell entry; single-particle; modeling; INFLUENZA-VIRUS HEMAGGLUTININ; CONFORMATIONAL-CHANGE; SINGLE-PARTICLE; FREE-ENERGY; TRANSMEMBRANE DOMAIN; FUSOGENIC ACTIVITY; HELICAL HAIRPIN; DISULFIDE BONDS; SIALIC-ACID; PH;
D O I
10.1146/annurev-biophys-070317-033018
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Influenza hemagglutinin (HA) is a viral membrane protein responsible for the initial steps of the entry of influenza virus into the host cell. It mediates binding of the virus particle to the host-cell membrane and catalyzes fusion of the viral membrane with that of the host. HA is therefore a major target in the development of antiviral strategies. The fusion of two membranes involves high activation barriers and proceeds through several intermediate states. Here, we provide a biophysical description of the membrane fusion process, relating its kinetic and thermodynamic properties to the large conformational changes taking place in HA and placing these in the context of multiple HA proteins working together to mediate fusion. Furthermore, we highlight the role of novel single-particle experiments and computational approaches in understanding the fusion process and their complementarity with other biophysical approaches.
引用
收藏
页码:153 / 173
页数:21
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