Exploring the Limits of Polyhydroxyalkanoate Production by Municipal Activated Sludge

被引：22
作者：
Pei, Ruizhe [1 ,2 ]
evez-Alonso, Angel Est [1 ,2 ]
Ortiz-Seco, Laura [2 ]
Loosdrecht, Mark C. M. van [1 ]
Kleerebezem, Robbert [1 ]
Werker, Alan [2 ]
机构：
[1] Delft Univ Technol, Dept Biotechnol, NL-2629 HZ Delft, Netherlands
[2] European Ctr Excellence Sustainable Water Technol, Wetsus, NL-8911 MA Leeuwarden, Netherlands
来源：
ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2022年 / 56卷 / 16期
基金：
欧盟地平线“2020”;
关键词：
resource recovery; municipal wastewater treatment; activated sludge; biopolymers; polyhydroxyalkanoate (PHA); WASTE-WATER TREATMENT; STORAGE POLYMERS; PHA PRODUCTION; CULTURE; REMOVAL; MICROBIOLOGY; TEMPERATURE; ENRICHMENT; RECOVERY; NITROGEN;
D O I：
10.1021/acs.est.2c03043
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Municipal activated sludge can be used for polyhydrox-yalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA-accumulating bacteria with respect to the total biomass), and (3) the average PHA content in the PHA-storing biomass fraction. The maximum attained biomass PHA content with different activated sludge ranged from 0.18 to 0.42 gPHA/gVSS, and the degree of enrichment ranged from 0.16 to 0.51 volume/volume. The average PHA content within the PHA-accumulating biomass fraction was relatively constant and independent of activated sludge source, with an average value of 0.58 & PLUSMN; 0.07 gPHA/gVSS. The degree of enrichment for PHA-accumulating bacteria was identified as the key factor to maximize PHA content when municipal activated sludge is directly used for PHA accumulation. Future optimization should focus on obtaining a higher degree of enrichment of PHA-accumulating biomass, either through selection during wastewater treatment or by selective growth during PHA accumulation. A PHA content in the order of 0.6 g PHA/g VSS is a realistic target to be achieved when using municipal activated sludge for PHA production.
引用
收藏
页码：11729 / 11738
页数：10
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