Online coupling of capillary electrophoresis with mass spectrometry for the identification of biomarkers for clinical diagnosis

被引：38
作者：
Weissinger, Eva M.
Hertenstein, Bernd
Mischak, Harald
Ganser, Arnold
机构：
[1] Hannover Med Sch, Dept Hematol Hemostasis & Oncol, D-30625 Hannover, Germany
[2] Klinikum Bremen Mitte GmbH, Med Klin 1, D-28177 Bremen, Germany
[3] Mosaiques Diagnost & Therapeut AG, Hannover, Germany
来源：
EXPERT REVIEW OF PROTEOMICS | 2005年 / 2卷 / 05期
关键词：
biomarker; capillary electrophoresis; clinical diagnosis; mass spectrometry; proteomics;
D O I：
10.1586/14789450.2.5.639
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteomic screening of complex biologic samples is of increasing importance in clinical research and diagnosis. In the postgenomic area it is evident that changes of the composition of body fluids, as well as post-translational modifications of proteins and peptides, provide more information than genetic typing. The study of these changes allows the state of health or disease of particular organs, and consequently, the whole organism, to be described. This review describes the application of capillary electrophoresis coupled online to an electrospray ionization time-of-flight mass spectrometer to the analysis of body fluids obtained from patients for the identification of biomarkers for diagnostic purposes.
引用
收藏
页码：639 / 647
页数：9
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