IGF binding protein-1 (IGFBP-1) is preferentially associated with the fetal facing basal surface of the syncytiotrophoblast in the human placenta

被引:5
作者
Fang, JG
Furesz, TC
Smith, CH
Fant, ME
机构
[1] Univ Texas, Sch Med, Div Neonatal Perinatal Med, Houston, TX 77030 USA
[2] Washington Univ, Sch Med, Dept Pediat, St Louis, MO USA
关键词
placenta; IGFBP-1; insulin-like growth factors; IGF binding proteins;
D O I
10.1054/ghir.1999.0127
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
IGF receptors are expressed in a spatially polarized manner on the syncytiotrophoblast cell membrane. We therefore examined the hypothesis that IGFBPs expressed at the maternal-fetal interface interact with distinct surfaces of the syncytiotrophoblast membrane to modulate IGF function. Membrane vesicles were prepared specifically from the maternal-facing, microvillous membrane (MVM) and the fetal-facing, basal membrane (BM) surfaces of the syncytiotrophoblast. The association of IGFBPs with each membrane preparation was determined by ligand blot analysis. A doublet migrating at 38/42 kD was detected in both MVM and BM preparations. Selective immunoprecipitation followed by ligand blot analysis identified this IGF binding species as IGFBP-3. Additionally, a protein migrating at approximately 29 kD was associated primarily with the BM. This protein was identified as IGFBP-1 by both immunoprecipitation and ligandblotting techniques. Non-denaturing PAGE revealed five distinct bands corresponding to different degrees of phosphorylation, The phosphorylation pattern of BM-associated IGFBP-1 was identical to that of native IGFBP-1 in amniotic fluid. Immunohistological analysis of term placenta revealed IGFBP-1-specific staining of the syncytiotrophoblast and the fetal capillary/pericapillary bed. The localization of IGFBP-1 to a distinct compartment within the fetal placenta, not in proximity to the syncytiotrophoblast type I IGF receptor, suggests it may play a role in regulating/targeting IGF activity within the stromal compartment or by exerting IGF-independent effects on the basal surface of the syncytiotrophoblast. The nature of its binding to the BM has not been determined. (C) 1999 Harcourt Publishers Ltd.
引用
收藏
页码:438 / 444
页数:7
相关论文
共 34 条
[1]   TRANSCAPILLARY PERMEABILITY AND SUBENDOTHELIAL DISTRIBUTION OF ENDOTHELIAL AND AMNIOTIC-FLUID INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS IN THE RAT-HEART [J].
BAR, RS ;
CLEMMONS, DR ;
BOES, M ;
BUSBY, WH ;
BOOTH, BA ;
DAKE, BL ;
SANDRA, A .
ENDOCRINOLOGY, 1990, 127 (03) :1078-1086
[2]   INSULIN DIFFERENTIALLY ALTERS TRANSCAPILLARY MOVEMENT OF INTRAVASCULAR IGFBP-1, IGFBP-2 AND ENDOTHELIAL-CELL IGF-BINDING PROTEINS IN THE RAT-HEART [J].
BAR, RS ;
BOES, M ;
CLEMMONS, DR ;
BUSBY, WH ;
SANDRA, A ;
DAKE, BL ;
BOOTH, BA .
ENDOCRINOLOGY, 1990, 127 (01) :497-499
[3]   2 IMMUNOREACTIVE BINDING-PROTEINS FOR INSULIN-LIKE GROWTH-FACTORS IN HUMAN AMNIOTIC-FLUID - RELATIONSHIP TO FETAL MATURITY [J].
BAXTER, RC ;
MARTIN, JL ;
WOOD, MH .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1987, 65 (03) :423-431
[4]   AN IMPROVED METHOD FOR THE PREPARATION OF HUMAN PLACENTAL SYNCYTIOTROPHOBLAST MICROVILLI [J].
BOOTH, AG ;
OLANIYAN, RO ;
VANDERPUYE, OA .
PLACENTA, 1980, 1 (04) :327-336
[5]   Insulin-like growth factor binding protein gene expression in the pregnant rat uterus and placenta [J].
Cerro, JA ;
Pintar, JE .
DEVELOPMENTAL BIOLOGY, 1997, 184 (02) :278-295
[6]   Role of insulin-like growth factor binding proteins in controlling IGF actions [J].
Clemmons, DR .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1998, 140 (1-2) :19-24
[7]   The pattern of expression of insulin-like growth factor (IGF), IGF-I receptor and IGF binding protein (IGFBP) mRNAs in the rhesus monkey placenta suggests a paracrine mode of IGF-IGFBP interaction in placental development [J].
Coulter, CL ;
Han, VKM .
PLACENTA, 1996, 17 (07) :451-460
[8]   Immunohistochemical analysis of insulin-like growth factor-binding proteins -1, -2, and -3 in implantation sites of the mouse [J].
Damario, MA ;
Liu, HC ;
Mele, CA ;
Horenstein, MG ;
Rosenwaks, Z .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1998, 15 (08) :513-520
[9]   Spatial polarization of insulin-like growth factor receptors on the human syncytiotrophoblast [J].
Fang, JG ;
Furesz, TC ;
Laurent, RS ;
Smith, CH ;
Fant, ME .
PEDIATRIC RESEARCH, 1997, 41 (02) :258-265
[10]   PRODUCTION OF INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN(S) (IGF-BPS) BY HUMAN-PLACENTA - VARIATION WITH GESTATIONAL-AGE [J].
FANT, M ;
MUNRO, H ;
MOSES, AC .
PLACENTA, 1988, 9 (04) :397-407