Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines

被引：45
|
作者：
El-Bahrawy, M
Poulsom, SR
Rowan, AJ
Tomlinson, IT
Alison, MR
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept Histopathol, London W12 0NN, England
[2] Canc Res UK, Dept Histopathol, London, England
[3] Canc Res UK, Mol & Populat Genet Lab, London, England
来源：
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2004年 / 85卷 / 02期
关键词：
catenins; colorectal carcinoma; E-cadherin;
D O I：
10.1111/j.0959-9673.2004.0371.x
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations.
引用
收藏
页码：65 / 74
页数：10
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