Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines

被引：48
作者：
El-Bahrawy, M
Poulsom, SR
Rowan, AJ
Tomlinson, IT
Alison, MR
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept Histopathol, London W12 0NN, England
[2] Canc Res UK, Dept Histopathol, London, England
[3] Canc Res UK, Mol & Populat Genet Lab, London, England
来源：
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2004年 / 85卷 / 02期
关键词：
catenins; colorectal carcinoma; E-cadherin;
D O I：
10.1111/j.0959-9673.2004.0371.x
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations.
引用
收藏
页码：65 / 74
页数：10
相关论文
共 53 条
[1]   beta-catenin is a target for the ubiquitin-proteasome pathway [J].
Aberle, H ;
Bauer, A ;
Stappert, J ;
Kispert, A ;
Kemler, R .
EMBO JOURNAL, 1997, 16 (13) :3797-3804
[2]  
ABERLE H, 1994, J CELL SCI, V107, P3655
[3]   Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion [J].
Adams, CL ;
Nelson, WJ ;
Smith, SJ .
JOURNAL OF CELL BIOLOGY, 1996, 135 (06) :1899-1911
[4]   Functional interaction of an axin homolog, conductin, with β-catenin, APC, and GSK3β [J].
Behrens, J ;
Jerchow, BA ;
Würtele, M ;
Grimm, J ;
Asbrand, C ;
Wirtz, R ;
Kühl, M ;
Wedlich, D ;
Birchmeier, W .
SCIENCE, 1998, 280 (5363) :596-599
[5]   LOCALIZATION OF THE GENE FOR FAMILIAL ADENOMATOUS POLYPOSIS ON CHROMOSOME-5 [J].
BODMER, WF ;
BAILEY, CJ ;
BODMER, J ;
BUSSEY, HJR ;
ELLIS, A ;
GORMAN, P ;
LUCIBELLO, FC ;
MURDAY, VA ;
RIDER, SH ;
SCAMBLER, P ;
SHEER, D ;
SOLOMON, E ;
SPURR, NK .
NATURE, 1987, 328 (6131) :614-616
[6]   E-CADHERIN EXPRESSION DURING THE ACIDIC FGF-INDUCED DISPERSION OF A RAT BLADDER-CARCINOMA CELL-LINE [J].
BOYER, B ;
DUFOUR, S ;
THIERY, JP .
EXPERIMENTAL CELL RESEARCH, 1992, 201 (02) :347-357
[7]   Armadillo and dTCF: a marriage made in the nucleus [J].
Cavallo, R ;
Rubenstein, D ;
Peifer, M .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1997, 7 (04) :459-466
[8]   Variable nuclear localization of α-catenin in colorectal carcinoma [J].
El-Bahrawy, M ;
Talbot, I ;
Poulsom, R ;
Alison, M .
LABORATORY INVESTIGATION, 2002, 82 (09) :1167-1174
[9]   The expression of E-cadherin and catenins in sporadic colorectal carcinoma [J].
El-Bahrawy, MA ;
Poulsom, R ;
Jeffery, R ;
Talbot, A ;
Alison, MR .
HUMAN PATHOLOGY, 2001, 32 (11) :1216-1224
[10]   The expression of E-cadherin and catenins in colorectal tumours from familial adenomatous polyposis patients [J].
El-Bahrawy, MA ;
Talbot, IC ;
Poulsom, R ;
Jeffery, R ;
Alison, MR .
JOURNAL OF PATHOLOGY, 2002, 198 (01) :69-76
123456