Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines

被引：45
作者：
El-Bahrawy, M
Poulsom, SR
Rowan, AJ
Tomlinson, IT
Alison, MR
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept Histopathol, London W12 0NN, England
[2] Canc Res UK, Dept Histopathol, London, England
[3] Canc Res UK, Mol & Populat Genet Lab, London, England
来源：
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2004年 / 85卷 / 02期
关键词：
catenins; colorectal carcinoma; E-cadherin;
D O I：
10.1111/j.0959-9673.2004.0371.x
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations.
引用
收藏
页码：65 / 74
页数：10
相关论文
共 53 条
  • [1] beta-catenin is a target for the ubiquitin-proteasome pathway
    Aberle, H
    Bauer, A
    Stappert, J
    Kispert, A
    Kemler, R
    [J]. EMBO JOURNAL, 1997, 16 (13) : 3797 - 3804
  • [2] ABERLE H, 1994, J CELL SCI, V107, P3655
  • [3] Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion
    Adams, CL
    Nelson, WJ
    Smith, SJ
    [J]. JOURNAL OF CELL BIOLOGY, 1996, 135 (06) : 1899 - 1911
  • [4] Functional interaction of an axin homolog, conductin, with β-catenin, APC, and GSK3β
    Behrens, J
    Jerchow, BA
    Würtele, M
    Grimm, J
    Asbrand, C
    Wirtz, R
    Kühl, M
    Wedlich, D
    Birchmeier, W
    [J]. SCIENCE, 1998, 280 (5363) : 596 - 599
  • [5] LOCALIZATION OF THE GENE FOR FAMILIAL ADENOMATOUS POLYPOSIS ON CHROMOSOME-5
    BODMER, WF
    BAILEY, CJ
    BODMER, J
    BUSSEY, HJR
    ELLIS, A
    GORMAN, P
    LUCIBELLO, FC
    MURDAY, VA
    RIDER, SH
    SCAMBLER, P
    SHEER, D
    SOLOMON, E
    SPURR, NK
    [J]. NATURE, 1987, 328 (6131) : 614 - 616
  • [6] E-CADHERIN EXPRESSION DURING THE ACIDIC FGF-INDUCED DISPERSION OF A RAT BLADDER-CARCINOMA CELL-LINE
    BOYER, B
    DUFOUR, S
    THIERY, JP
    [J]. EXPERIMENTAL CELL RESEARCH, 1992, 201 (02) : 347 - 357
  • [7] Armadillo and dTCF: a marriage made in the nucleus
    Cavallo, R
    Rubenstein, D
    Peifer, M
    [J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 1997, 7 (04) : 459 - 466
  • [8] Variable nuclear localization of α-catenin in colorectal carcinoma
    El-Bahrawy, M
    Talbot, I
    Poulsom, R
    Alison, M
    [J]. LABORATORY INVESTIGATION, 2002, 82 (09) : 1167 - 1174
  • [9] The expression of E-cadherin and catenins in sporadic colorectal carcinoma
    El-Bahrawy, MA
    Poulsom, R
    Jeffery, R
    Talbot, A
    Alison, MR
    [J]. HUMAN PATHOLOGY, 2001, 32 (11) : 1216 - 1224
  • [10] The expression of E-cadherin and catenins in colorectal tumours from familial adenomatous polyposis patients
    El-Bahrawy, MA
    Talbot, IC
    Poulsom, R
    Jeffery, R
    Alison, MR
    [J]. JOURNAL OF PATHOLOGY, 2002, 198 (01) : 69 - 76
123456