Identification and Characterization of Mycemycin Biosynthetic Gene Clusters in Streptomyces olivaceus FXJ8.012 and Streptomyces sp FXJ1.235

被引：4

作者：

Song, Fangying ^{[1
,2
]}

Liu, Ning ^{[1
]}

Liu, Minghao ^{[1
]}

Chen, Yihua ^{[1
]}

Huang, Ying ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

来源：

MARINE DRUGS | 2018年 / 16卷 / 03期

基金：

中国国家自然科学基金;

关键词：

mycemycin; dibenzoxazepinone; biosynthesis; gene cluster; pathway; Streptomyces; TRYPTOPHAN; DIBENZOXAZEPINONES; CONSTRUCTION; PATHWAY; ACID; DNA;

D O I：

10.3390/md16030098

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Mycemycins A-E are new members of the dibenzoxazepinone (DBP) family, derived from the gntR gene-disrupted deep sea strain Streptomyces olivaceus FXJ8.012 Delta 1741 and the soil strain Streptomyces sp. FXJ1.235. In this paper, we report the identification of the gene clusters and pathways' inference for mycemycin biosynthesis in the two strains. Bioinformatics analyses of the genome sequences of S. olivaceus FXJ8.012 Delta 1741 and S. sp. FXJ1.235 predicted two divergent mycemycin gene clusters, mym and mye, respectively. Heterologous expression of the key enzyme genes of mym and genetic manipulation of mye as well as a feeding study in S. sp. FXJ1.235 confirmed the gene clusters and led to the proposed biosynthetic pathways for mycemycins. To the best of our knowledge, this is the first report on DBP biosynthetic gene clusters and pathways.

引用

页数：12

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