Assessment of the Campylobacter jejuni and C. coil in broiler chicken ceca by conventional culture and loop-mediated isothermal amplification method

被引：6
作者：
Sabike, Islam Ibrahim [1 ,4 ]
Uemura, Ryoko [1 ,2 ]
Kirino, Yumi [2 ]
Mekata, Hirohisa [2 ,3 ]
Sekiguchi, Satoshi [1 ,2 ]
Farid, Ayman Samir [5 ]
Goto, Yoshitaka [1 ,2 ]
Horii, Yoichiro [1 ,2 ]
Yamazaki, Wataru [1 ,2 ]
机构：
[1] Miyazaki Univ, Dept Vet Sci, Fac Agr, Miyazaki, Japan
[2] Miyazaki Univ, Ctr Anim Dis Control, Miyazaki, Japan
[3] Miyazaki Univ, Org Promot Tenure Track, Miyazaki, Japan
[4] Benha Univ, Dept Food Control, Fac Vet Med, Banha, Egypt
[5] Benha Univ, Dept Clin Pathol, Fac Vet Med, Banha, Egypt
来源：
FOOD CONTROL | 2017年 / 74卷
关键词：
Food poisoning; Prevalence; Campylobacter; Seasonal change; Broiler chicken ceca; LAMP; RISK-FACTORS; COLONIZATION; SALMONELLA; FLOCKS; ASSAY; SLAUGHTER; RECOVERY; FARM; SPP; COLI;
D O I：
10.1016/j.foodcont.2016.11.037
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In a two-year survey of the 24 Japanese broiler chicken flocks at 9 farms from 2013 to 2014, C. jejuni/C. colt prevalence was assessed in a total of 131 slaughtered broiler chicken cecal samples by conventional culture methods and loop-mediated isothermal amplification (LAMP) assay. While 93 samples were C. jejuni/C. coli-negative, 38 (29.0%) showed Campylobacter loads of between 6.4 and 9.0 log CFU/g of ceca in conventional culture methods. The performance of LAMP assay was 100% accurate in terms of diagnostic sensitivity (38/38), specificity (93/93). Furthermore, LAMP assay enabled direct screening of C. jejuni and C. colt in cecal samples from broiler chicken chickens as rapid and cost-effective detection within 90 min and less than 1 US dollar, which can help monitor release of Campylobacter-contamitiated chicken into the food chain, thereby reducing the incidence and public health risk of campylobacteriosis. Seasonal changes in C. jejuni and C. coli prevalence in broiler chicken ceca were significantly correlated with the frequency of food poisoning incidents caused by these bacteria in Japan. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：107 / 111
页数：5
相关论文
共 19 条
  • [1] Prevalence and risk factors for Salmonella spp. and Campylobacter spp. caecal colonization in broiler chicken and turkey flocks slaughtered in Quebec, Canada
    Arsenault, Julie
    Letellier, Ann
    Quessy, Sylvain
    Normand, Valerie
    Boulianne, Martine
    [J]. PREVENTIVE VETERINARY MEDICINE, 2007, 81 (04) : 250 - 264
  • [2] Campylobacter incidence on a chicken farm and the spread of Campylobacter during the slaughter process
    Berndtson, E
    DanielssonTham, ML
    Engvall, A
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 32 (1-2) : 35 - 47
  • [3] Campylobacter recovery from external and internal organs of commercial broiler carcass prior to scalding
    Berrang, ME
    Buhr, RJ
    Cason, JA
    [J]. POULTRY SCIENCE, 2000, 79 (02) : 286 - 290
  • [4] Incidence of Campylobacter in crops of preharvest market-age broiler chickens
    Byrd, JA
    Corrier, DE
    Hume, ME
    Bailey, RH
    Stanker, LH
    Hargis, BM
    [J]. POULTRY SCIENCE, 1998, 77 (09) : 1303 - 1305
  • [5] CECAL CARRIAGE OF CAMPYLOBACTER AND SALMONELLA IN DUTCH BROILER FLOCKS AT SLAUGHTER - A ONE-YEAR STUDY
    JACOBSREITSMA, WF
    BOLDER, NM
    MULDER, RWAW
    [J]. POULTRY SCIENCE, 1994, 73 (08) : 1260 - 1266
  • [6] Effect of climate and farm environment on Campylobacter spp. colonisation in Norwegian broiler flocks
    Jonsson, Malin E.
    Chriel, Mariann
    Norstrom, Madelaine
    Hofshagen, Merete
    [J]. PREVENTIVE VETERINARY MEDICINE, 2012, 107 (1-2) : 95 - 104
  • [7] Influence of Season and Geography on Campylobacter jejuni and C. coli Subtypes in Housed Broiler Flocks Reared in Great Britain
    Jorgensen, F.
    Ellis-Iversen, J.
    Rushton, S.
    Bull, S. A.
    Harris, S. A.
    Bryan, S. J.
    Gonzalez, A.
    Humphrey, T. J.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (11) : 3741 - 3748
  • [8] EPIDEMIOLOGIC INVESTIGATION OF RISK-FACTORS FOR CAMPYLOBACTER COLONIZATION IN NORWEGIAN BROILER FLOCKS
    KAPPERUD, G
    SKJERVE, E
    VIK, L
    HAUGE, K
    LYSAKER, A
    AALMEN, I
    OSTROFF, SM
    POTTER, M
    [J]. EPIDEMIOLOGY AND INFECTION, 1993, 111 (02) : 245 - 255
  • [9] Predominant Campylobacter jejuni Sequence Types Persist in Finnish Chicken Production
    Llarena, Ann-Katrin
    Huneau, Adeline
    Hakkinen, Marjaana
    Hanninen, Marja-Liisa
    [J]. PLOS ONE, 2015, 10 (02):
  • [10] Loop-mediated isothermal amplification of DNA
    Notomi, Tsugunori
    Okayama, Hiroto
    Masubuchi, Harumi
    Yonekawa, Toshihiro
    Watanabe, Keiko
    Amino, Nobuyuki
    Hase, Tetsu
    [J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (12)
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