Toosendanin increases free-Ca2+ concentration in NG108-15 cells via L-type Ca2+ channels

被引：0

作者：

Xu, TH ^{[1
]}

Ding, J ^{[1
]}

Shi, YL ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Neurobiol, Shanghai 200031, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2004年 / 25卷 / 05期

关键词：

toosendanin; intracellular calcium concentration; confocal microscopy; calcium indicator; neuroblastomaxglioma hybrid cells; L-type Ca2+ channels;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+](i)) in neuroblastomaxglioma hybrid cells (NG108-15 cells). METHODS: The [Ca2+](i) was determined by laser-scanning confocal microscopic imaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting [Ca2+](i) and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+](i) was dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared after washing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+](i) in differentiated NG108-15 cells. The [Ca2+](i) enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+ channels.

引用

页码：597 / 601

页数：5

共 18 条

[11]

SHEN GG, 1994, ACTA PHYSL SIN, V46, P546

[12]

Shi Y.L., 1981, Acta Physiol. Sin., V33, P259

[13]

SHI YL, 1993, CHINESE SCI BULL, V38, P1489

[14] Effect of Toosendanin on acetylcholine level of rat brain, a microdialysis study [J].

Shi, YL ;

Chen, WY .

BRAIN RESEARCH, 1999, 850 (1-2) :173-178

[15]

SHI YL, 1982, ACTA PHYSL SIN, V34, P304

[16]

SHIN YL, 1983, JPN J PHYSL, V33, P677

[17]

Shu G.-X., 1980, ACTA CHIM SINICA, V38, P196

[18] Caffeine-sensitive Ca2+ stores in carp retinal bipolar cells [J].

Wu, D ;

Zhu, PH .

NEUROREPORT, 1999, 10 (18) :3897-3901

← 1 2 →