Expression of Epithelial Cell Adhesion Molecule (EpCAM) in Tumor Spheroids of Human Colorectal Adenocarcinoma Cells

被引：2

作者：

Gisina, A. M. ^{[1
]}

Kim, Ya. S. ^{[1
]}

Gabashvili, A. N. ^{[2
]}

Tsvetkova, A. V. ^{[1
]}

Vakhrushev, I. V. ^{[1
]}

Yarygin, K. N. ^{[1
]}

Lupatov, A. Yu. ^{[1
]}

机构：

[1] VN Orekhovich Res Inst Biomed Chem, Moscow, Russia

[2] Natl Univ Sci & Technol MISIS, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2020年 / 170卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

tumor spheroids; colorectal cancer; HCT116; surface markers; EpCAM; E-CADHERIN; CULTURE; ANTIGEN; MARKERS;

D O I：

10.1007/s10517-020-05018-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the formation of spheroids by Caco-2, SW480, and HCT116 human colorectal adenocarcinoma cell lines under low-adhesion culturing conditions. Of these three cell lines, only HCT116 formed stable tumor spheroids. Flow cytometry analysis of 19 surface markers in monolayer HCT116 culture and spheroids formed by these cells revealed considerable similarity of the expression profiles in these two culturing modes. The only exception was EpCAM molecule: its expression in spheroids was 3-fold higher than in the monolayer culture. Scanning confocal laser microscopy showed equal EpCAM distribution in the inner mass of the spheroids.

引用

页码：135 / 141

页数：7

共 50 条

[31] Epithelial Cell Adhesion Molecule Expression in Canine Tumours [J].

Thamm, D. H. ;

Hayes, D. F. ;

Meuten, T. ;

Laver, T. ;

Thomas, D. G. .

JOURNAL OF COMPARATIVE PATHOLOGY, 2016, 155 (04) :299-304

[32] Epithelial Cell Adhesion Molecule (EpCAM) Complex Proteins Promote Transcription Factor-mediated Pluripotency Reprogramming [J].

Huang, Hsiang-Po ;

Chen, Pin-Hsun ;

Yu, Chun-Ying ;

Chuang, Ching-Yu ;

Stone, Lee ;

Hsiao, Wen-Chu ;

Li, Chung-Leung ;

Tsai, Shih-Chih ;

Chen, Kai-Yun ;

Chen, Hsin-Fu ;

Ho, Hong-Nerng ;

Kuo, Hung-Chih .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (38) :33520-33532

[33] Epithelial cell adhesion molecule fragments and signaling in primary human liver cells [J].

Gerlach, Jorg C. ;

Foka, Hubert G. ;

Thompson, Robert L. ;

Gridelli, Bruno ;

Schmelzer, Eva .

JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (06) :4841-4851

[34] Epithelial Cell Adhesion Molecule (EpCAM) Expression in Human Tumors: A Comparison with Pan-Cytokeratin and TROP2 in 14,832 Tumors [J].

Menz, Anne ;

Lony, Nora ;

Lennartz, Maximilian ;

Dwertmann Rico, Sebastian ;

Schlichter, Ria ;

Kind, Simon ;

Reiswich, Viktor ;

Viehweger, Florian ;

Dum, David ;

Luebke, Andreas M. ;

Kluth, Martina ;

Gorbokon, Natalia ;

Hube-Magg, Claudia ;

Bernreuther, Christian ;

Simon, Ronald ;

Clauditz, Till S. ;

Sauter, Guido ;

Hinsch, Andrea ;

Jacobsen, Frank ;

Marx, Andreas H. ;

Steurer, Stefan ;

Minner, Sarah ;

Burandt, Eike ;

Krech, Till ;

Lebok, Patrick ;

Weidemann, Soeren .

DIAGNOSTICS, 2024, 14 (10)

[35] The Epithelial Cell Adhesion Molecule EpCAM Is Required for Epithelial Morphogenesis and Integrity during Zebrafish Epiboly and Skin Development [J].

Slanchev, Krasimir ;

Carney, Thomas J. ;

Stemmler, Marc P. ;

Koschorz, Birgit ;

Amsterdam, Adam ;

Schwarz, Heinz ;

Hammerschmidt, Matthias .

PLOS GENETICS, 2009, 5 (07)

[36] Epithelial cell adhesion molecule (EpCAM) is involved in prostate cancer chemotherapy/radiotherapy response in vivo [J].

Ni, Jie ;

Cozzi, Paul ;

Beretov, Julia ;

Duan, Wei ;

Bucci, Joseph ;

Graham, Peter ;

Li, Yong .

BMC CANCER, 2018, 18

[37] Expression of Epithelial Cell Adhesion Molecule in Paired Tumor Samples of Patients With Primary and Recurrent Serous Ovarian Cancer [J].

Pietzner, Klaus ;

Woopen, Hannah ;

Richter, Rolf ;

Joens, Thomas ;

Braicu, Elena Ioana ;

Dimitrova, Desislava ;

Mellstedt, Hakan ;

Darb-Esfahani, Silvia ;

Denkert, Carsten ;

Lindhofer, Horst ;

Fotopoulou, Christina ;

Sehouli, Jalid .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2013, 23 (05) :797-802

[38] Cleavage and Cell Adhesion Properties of Human Epithelial Cell Adhesion Molecule (HEPCAM) [J].

Tsaktanis, Thanos ;

Kremling, Heidi ;

Pavslc, Miha ;

von Stackelberg, Ricarda ;

Mack, Brigitte ;

Fukumori, Akio ;

Steiner, Harald ;

Vielmuth, Franziska ;

Spindler, Volker ;

Huang, Zhe ;

Jakubowski, Jasmine ;

Stoecklein, Nikolas H. ;

Luxenburger, Elke ;

Lauber, Kirsten ;

Lenarcic, Brigita ;

Gires, Olivier .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (40) :24574-24591

[39] Clinical significance of serum epithelial cell adhesion molecule (EPCAM) levels in patients with lung cancer [J].

Tas, Faruk ;

Karabulut, Senem ;

Duranyildiz, Derya .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2014, 396 (1-2) :307-312

[40] Clinical significance of serum epithelial cell adhesion molecule (EPCAM) levels in patients with lung cancer [J].

Faruk Tas ;

Senem Karabulut ;

Derya Duranyildiz .

Molecular and Cellular Biochemistry, 2014, 396 :307-312

← 1 2 3 4 5 →