Design and performance of an ultra-flexible two-photon microscope for in vivo research

被引：53

作者：

Mayrhofer, Johannes M. ^{[1
,2
]}

Haiss, Florent ^{[1
,2
,3
,4
,5
]}

Haenni, Dominik ^{[6
,7
]}

Weber, Stefan ^{[1
]}

Zuend, Marc ^{[1
,2
]}

Barrett, Matthew J. P. ^{[1
,2
]}

Ferrari, Kim David ^{[1
,2
]}

Maechler, Philipp ^{[1
,2
]}

Saab, Aiman S. ^{[1
,2
]}

Stobart, Jillian L. ^{[1
,2
]}

Wyss, Matthias T. ^{[1
,2
]}

Johannssen, Helge ^{[1
,2
]}

Osswald, Harald ^{[1
]}

Palmer, Lucy M. ^{[8
,9
]}

Revol, Vincent ^{[10
]}

Schuh, Claus-Dieter ^{[7
]}

Urban, Claus ^{[10
]}

Hall, Andrew ^{[7
]}

Larkum, Matthew E. ^{[9
]}

Rutz-Innerhofer, Edith ^{[10
]}

Zeilhofer, Hanns Ulrich ^{[1
,2
]}

Ziegler, Urs ^{[6
]}

Weber, Bruno ^{[1
,2
]}

机构：

[1] Univ Zurich, Inst Pharmacol & Toxicol, CH-8057 Zurich, Switzerland

[2] Neurosci Ctr Zurich, CH-8057 Zurich, Switzerland

[3] Rhein Westfal TH Aachen, Fac Med, IZKF Aachen, D-52074 Aachen, Germany

[4] Rhein Westfal TH Aachen, Inst Neuropathol, D-52074 Aachen, Germany

[5] Rhein Westfal TH Aachen, Dept Ophthalmol, D-52074 Aachen, Germany

[6] Univ Zurich, Ctr Microscopy & Image Anal, CH-8057 Zurich, Switzerland

[7] Univ Zurich, Inst Anat, CH-8057 Zurich, Switzerland

[8] Univ Melbourne, Florey Inst, Melbourne, Vic 3010, Australia

[9] Charite, Neurosci Res Ctr, D-10117 Berlin, Germany

[10] CSEM Suisse Elect & Microtech, CH-8005 Zurich, Switzerland

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 11期

基金：

瑞士国家科学基金会;

关键词：

GABAERGIC NEURONS; POPULATIONS; EXCITATION; CORTEX; AWAKE; LIGHT; MICE;

D O I：

10.1364/BOE.6.004228

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a cost-effective in vivo two-photon microscope with a highly flexible frontend for in vivo research. Our design ensures fast and reproducible access to the area of interest, including rotation of imaging plane, and maximizes space for auxiliary experimental equipment in the vicinity of the animal. Mechanical flexibility is achieved with large motorized linear stages that move the objective in the X, Y, and Z directions up to 130 mm. 360 degrees rotation of the frontend (rotational freedom for one axis) is achieved with the combination of a motorized high precision bearing and gearing. Additionally, the modular design of the frontend, based on commercially available optomechanical parts, allows straightforward updates to future scanning technologies. The design exceeds the mobility of previous movable microscope designs while maintaining high optical performance. (C) 2015 Optical Society of America

引用

页码：4228 / 4237

页数：10