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Effects of GP2 expression on secretion and endocytosis in pancreatic AR4-2J cells
被引:8
|作者:
Yu, S
[1
]
Hao, Y
[1
]
Lowe, AW
[1
]
机构:
[1] Stanford Univ, Dept Med & Digest Dis Ctr, Stanford, CA USA
关键词:
pancreas;
exocrine;
membrane protein;
secretion;
secretory granule;
secretory pathway;
endocytosis;
GP2;
D O I:
10.1016/j.bbrc.2004.07.120
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
GP2 is the major membrane protein present in secretory granules of the exocrine pancreas. GP2's function is unknown, but a role in digestive enzyme packaging or secretion from secretory granules has been proposed. In addition, GP2 has been proposed to influence endocytosis and membrane recycling following stimulated secretion. Adenovirus-mediated GP2 overexpression in the rat pancreatic cell line AR4-2J was used to study its impact on digestive enzyme secretion and membrane recycling. Immunoelectron microscopy showed that GP2 and amylase co-localized in secretory granules in infected AR4-2J cells. CCK-8 stimulation resulted in a fourfold increase in amylase secretion with or without GP2 expression. GP2 expression also did not influence endocytosis following CCK-8 stimulation. Thus, GP2 expression in AR4-2J cells does not affect amylase packaging in secretory granules or stimulated secretion. GP2 expression also does not influence membrane recycling in response to stimulated stimulation in AR4-2J cells. (C) 2004 Elsevier Inc. All rights reserved.
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页码:320 / 325
页数:6
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