Effects of GP2 expression on secretion and endocytosis in pancreatic AR4-2J cells

被引:8
|
作者
Yu, S [1 ]
Hao, Y [1 ]
Lowe, AW [1 ]
机构
[1] Stanford Univ, Dept Med & Digest Dis Ctr, Stanford, CA USA
关键词
pancreas; exocrine; membrane protein; secretion; secretory granule; secretory pathway; endocytosis; GP2;
D O I
10.1016/j.bbrc.2004.07.120
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
GP2 is the major membrane protein present in secretory granules of the exocrine pancreas. GP2's function is unknown, but a role in digestive enzyme packaging or secretion from secretory granules has been proposed. In addition, GP2 has been proposed to influence endocytosis and membrane recycling following stimulated secretion. Adenovirus-mediated GP2 overexpression in the rat pancreatic cell line AR4-2J was used to study its impact on digestive enzyme secretion and membrane recycling. Immunoelectron microscopy showed that GP2 and amylase co-localized in secretory granules in infected AR4-2J cells. CCK-8 stimulation resulted in a fourfold increase in amylase secretion with or without GP2 expression. GP2 expression also did not influence endocytosis following CCK-8 stimulation. Thus, GP2 expression in AR4-2J cells does not affect amylase packaging in secretory granules or stimulated secretion. GP2 expression also does not influence membrane recycling in response to stimulated stimulation in AR4-2J cells. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:320 / 325
页数:6
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