Cloning, embryonic expression, and functional characterization of two novel connexins from Xenopus laevis

被引：5
作者：
de Boer, Teun P.
Kok, Bart
Roel, Giulietta
van Veen, Toon A. B.
Destree, Olivier H. J.
Rook, Martin B.
Vos, Marc A.
de Bakker, Jacques M. T.
van der Heyden, Marcel A. G.
机构：
[1] Univ Utrecht, Med Ctr, HLCU, Dept Med Physiol, NL-3584 CM Utrecht, Netherlands
[2] Netherlands Inst Dev Biol, Hubrecht Lab, NL-3584 CT Utrecht, Netherlands
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 349卷 / 02期
关键词：
connexin; phylogeny; gap junction; Xenopus; expression; electrophysiology; organizer; pronephros; endoderm; mesoderm; cement gland; liver anlage;
D O I：
10.1016/j.bbrc.2006.08.121
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Vertebrate gap junctions are constituted of connexin (Cx) proteins. In Xenopus laevis, only seven different Cxs have been described so far. Here, we identify two new Cxs from X. laevis. Cx28.6 displays > 60% amino acid identity with human Cx25, Cx29 displays strong homology with mouse Cx26 and Cx30. Cx29 is expressed throughout embryonic development. Cx28.6 mRNA is only transiently found from stage 22 to 26 of development. While no Cx28.6 expression could be detected by whole mount in situ hybridization, expression of Cx29 was found in the developing endoderm, lateral mesoderm, liver anlage, pronephros, and proctodeum. Ectopic expression of Cx28.6 failed to produce functional gap-junctions. In contrast, ectopic expression of full-length Cx29 in HEK293 and COS-7 cells resulted in the formation of gap junction-like structures at the cell-cell interfaces. Ectopic expression of Cx29 in communication deficient N2A cell pairs led to functional electrical coupling. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：855 / 862
页数：8
相关论文
共 26 条
[1]   GAP-JUNCTIONS FORMED BY CONNEXIN-26 AND CONNEXIN-32 ALONE AND IN COMBINATION ARE DIFFERENTLY AFFECTED BY APPLIED VOLTAGE [J].
BARRIO, LC ;
SUCHYNA, T ;
BARGIELLO, T ;
XU, LX ;
ROGINSKI, RS ;
BENNETT, MVL ;
NICHOLSON, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8410-8414
[2]   Cloning and functional expression of a novel human connexin-25 gene [J].
Bondarev, I ;
Vine, A ;
Bertram, JS .
CELL COMMUNICATION AND ADHESION, 2001, 8 (4-6) :167-+
[3]   Connections with connexins: The molecular basis of direct intercellular signaling [J].
Bruzzone, R ;
White, TW ;
Paul, DL .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 238 (01) :1-27
[4]   The vertebrate connexin family [J].
Cruciani, V. ;
Mikalsen, S. -O. .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2006, 63 (10) :1125-1140
[5]   The connexin gene family in mammals [J].
Cruciani, W ;
Mikalsen, SO .
BIOLOGICAL CHEMISTRY, 2005, 386 (04) :325-332
[6]   Xenopus connexins:: how frogs bridge the gap [J].
de Boer, TP ;
van der Heyden, MAG .
DIFFERENTIATION, 2005, 73 (07) :330-340
[7]   Cloning and functional characterization of a novel connexin expressed in somites of Xenopus laevis [J].
De Boer, TP ;
Kok, B ;
Neuteboom, KIE ;
Spicker, N ;
De Graaf, J ;
Destrée, OHJ ;
Rook, MB ;
Van Veen, TAB ;
Jongsma, HJ ;
Vos, MA ;
De Bakker, JMT ;
Van Der Heyden, MAG .
DEVELOPMENTAL DYNAMICS, 2005, 233 (03) :864-871
[8]  
HARLAND RM, 1991, METHOD CELL BIOL, V36, P685
[9]   Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes [J].
Krogh, A ;
Larsson, B ;
von Heijne, G ;
Sonnhammer, ELL .
JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (03) :567-580
[10]   MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].
Kumar, S ;
Tamura, K ;
Nei, M .
BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163
123